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Literature summary for 1.14.14.158 extracted from

  • Chang, S.; Berman, J.; Sheng, Y.; Wang, Y.; Capell, T.; Shi, L.; Ni, X.; Sandmann, G.; Christou, P.; Zhu, C.
    Cloning and functional characterization of the maize (Zea mays L.) carotenoid epsilon hydroxylase gene (2015), PLoS ONE, 10, e0128758 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of CYP97C19 cDNA under the control of the constitutive CaMV 35S promoter in the Arabidopsis thaliana lut1 knockout mutant, which lacks a functional CYP97C1 (LUT1) gene Zea mays
gene CYP97C19, cDNA is cloned from leaves, the ZmCYP97C19 gene has nine introns and ten exons, DNA and amino acid sequence determination and analysis, sequence comparisons, genetic structure, overview. Functional recombinant expression under the control of constitutive CaMV 35S promoter in rosettes leaves of the Arabidopsis thaliana lut1 knockout mutant, which lacks a functional CYP97C1 (LUT1) gene. Lutein accumulates to high levels in the rosette leaves of the transgenic lines but not in the untransformed lut1 mutants, carotenoid extraction and quantification from Arabidopsis thaliana lut1-CYP97C1 leaves Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
zeinoxanthin + [reduced NADPH-hemoprotein reductase] + O2 Zea mays maize CYP97C19 is an enzyme with strong zeinoxanthin epsilon-ring hydroxylation activity lutein + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
zeinoxanthin + [reduced NADPH-hemoprotein reductase] + O2 Zea mays L. cv B73 maize CYP97C19 is an enzyme with strong zeinoxanthin epsilon-ring hydroxylation activity lutein + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Organism

Organism UniProt Comment Textmining
Zea mays K9J9Z8
-
-
Zea mays K9J9Z8 cv. B73
-
Zea mays L. cv B73 K9J9Z8
-
-

Source Tissue

Source Tissue Comment Organism Textmining
endosperm
-
Zea mays
-
leaf
-
Zea mays
-
rosette leaf
-
Zea mays
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
zeinoxanthin + [reduced NADPH-hemoprotein reductase] + O2 maize CYP97C19 is an enzyme with strong zeinoxanthin epsilon-ring hydroxylation activity Zea mays lutein + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
zeinoxanthin + [reduced NADPH-hemoprotein reductase] + O2 maize CYP97C19 is an enzyme with strong zeinoxanthin epsilon-ring hydroxylation activity Zea mays L. cv B73 lutein + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Subunits

Subunits Comment Organism
? x * 61900, about, sequence calculation of ZmCYP97C19 Zea mays

Synonyms

Synonyms Comment Organism
carotenoid epsilon hydroxylase
-
Zea mays
carotenoid epsilon-hydroxylase
-
Zea mays
CYP97C
-
Zea mays
CYP97C19
-
Zea mays
heme-containing cytochrome P450 carotene epsilon-ring hydroxylase
-
Zea mays
LUT1
-
Zea mays

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
-
Zea mays
heme
-
Zea mays