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Literature summary for 1.14.14.155 extracted from

  • Kadow, M.; Loschinski, K.; Sass, S.; Schmidt, M.; Bornscheuer, U.
    Completing the series of BVMOs involved in camphor metabolism of Pseudomonas putida NCIMB 10007 by identification of the two missing genes, their functional expression in E. coli, and biochemical characterization (2012), Appl. Microbiol. Biotechnol., 96, 419-429.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
x * 40000, SDS-PAGE Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida D7UER1
-
-
Pseudomonas putida NCIMB 10007 D7UER1
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.09
-
crude extract, at pH 7.5 and 25°C Pseudomonas putida
0.46
-
purified enzyme, at pH 7.5 and 25°C Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-camphor + FMNH2 + O2 88% conversion Pseudomonas putida ? + FMN + H2O
-
?
(+)-camphor + FMNH2 + O2 88% conversion Pseudomonas putida NCIMB 10007 ? + FMN + H2O
-
?
(+/-)-cis-bicyclo[3.2.0]hept-2-en-6-one + FMNH2 + O2 99% conversion Pseudomonas putida ?
-
?
(+/-)-cis-bicyclo[3.2.0]hept-2-en-6-one + FMNH2 + O2 99% conversion Pseudomonas putida NCIMB 10007 ?
-
?
(-)-camphor + FMNH2 + O2 91% conversion Pseudomonas putida ? + FMN + H2O
-
?
(-)-camphor + FMNH2 + O2 91% conversion Pseudomonas putida NCIMB 10007 ? + FMN + H2O
-
?
(R,R)-bicyclo[2.2.1]heptane-2,5-dione + FMNH2 + O2 26% conversion Pseudomonas putida ?
-
?
(R,R)-bicyclo[2.2.1]heptane-2,5-dione + FMNH2 + O2 26% conversion Pseudomonas putida NCIMB 10007 ?
-
?
2,3,4,5-tetramethyl-2-cyclopenten-1-one + FMNH2 + O2 43% conversion Pseudomonas putida ?
-
?
2,3,4,5-tetramethyl-2-cyclopenten-1-one + FMNH2 + O2 43% conversion Pseudomonas putida NCIMB 10007 ?
-
?
2-cyclohexen-1-one + FMNH2 + O2 50% conversion Pseudomonas putida ?
-
?
2-cyclopenten-1-one + FMNH2 + O2 48% conversion Pseudomonas putida ?
-
?
2-decanone + FMNH2 + O2 11% conversion Pseudomonas putida ?
-
?
3,5,5-trimethyl-2-cyclohexen-1-one + FMNH2 + O2 21% conversion Pseudomonas putida ?
-
?
3-methyl-2-cyclohexen-1-one + FMNH2 + O2 20% conversion Pseudomonas putida ?
-
?
3-methyl-2-cyclopenten-1-one + FMNH2 + O2 10% conversion Pseudomonas putida ?
-
?
4-phenyl-2-butanone + FMNH2 + O2 48% conversion Pseudomonas putida ?
-
?
acetophenone + FMNH2 + O2 80% conversion Pseudomonas putida ?
-
?
cyclobutanone + FMNH2 + O2 13% conversion Pseudomonas putida ?
-
?
cyclohexanone + FMNH2 + O2 3% conversion Pseudomonas putida ?
-
?
cyclopentanone + FMNH2 + O2 24% conversion Pseudomonas putida ?
-
?
norcamphor + FMNH2 + O2 77% conversion Pseudomonas putida ?
-
?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Pseudomonas putida

Synonyms

Synonyms Comment Organism
3,6-diketocamphane 1,6-monooxygenase
-
Pseudomonas putida
3,6-DKCMO
-
Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
FMN dependent on Pseudomonas putida
additional information dependent on Pseudomonas putida