Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SDS-PAGE | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | D7UER1 | - |
- |
Pseudomonas putida NCIMB 10007 | D7UER1 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.09 | - |
crude extract, at pH 7.5 and 25°C | Pseudomonas putida |
0.46 | - |
purified enzyme, at pH 7.5 and 25°C | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-camphor + FMNH2 + O2 | 88% conversion | Pseudomonas putida | ? + FMN + H2O | - |
? | |
(+)-camphor + FMNH2 + O2 | 88% conversion | Pseudomonas putida NCIMB 10007 | ? + FMN + H2O | - |
? | |
(+/-)-cis-bicyclo[3.2.0]hept-2-en-6-one + FMNH2 + O2 | 99% conversion | Pseudomonas putida | ? | - |
? | |
(+/-)-cis-bicyclo[3.2.0]hept-2-en-6-one + FMNH2 + O2 | 99% conversion | Pseudomonas putida NCIMB 10007 | ? | - |
? | |
(-)-camphor + FMNH2 + O2 | 91% conversion | Pseudomonas putida | ? + FMN + H2O | - |
? | |
(-)-camphor + FMNH2 + O2 | 91% conversion | Pseudomonas putida NCIMB 10007 | ? + FMN + H2O | - |
? | |
(R,R)-bicyclo[2.2.1]heptane-2,5-dione + FMNH2 + O2 | 26% conversion | Pseudomonas putida | ? | - |
? | |
(R,R)-bicyclo[2.2.1]heptane-2,5-dione + FMNH2 + O2 | 26% conversion | Pseudomonas putida NCIMB 10007 | ? | - |
? | |
2,3,4,5-tetramethyl-2-cyclopenten-1-one + FMNH2 + O2 | 43% conversion | Pseudomonas putida | ? | - |
? | |
2,3,4,5-tetramethyl-2-cyclopenten-1-one + FMNH2 + O2 | 43% conversion | Pseudomonas putida NCIMB 10007 | ? | - |
? | |
2-cyclohexen-1-one + FMNH2 + O2 | 50% conversion | Pseudomonas putida | ? | - |
? | |
2-cyclopenten-1-one + FMNH2 + O2 | 48% conversion | Pseudomonas putida | ? | - |
? | |
2-decanone + FMNH2 + O2 | 11% conversion | Pseudomonas putida | ? | - |
? | |
3,5,5-trimethyl-2-cyclohexen-1-one + FMNH2 + O2 | 21% conversion | Pseudomonas putida | ? | - |
? | |
3-methyl-2-cyclohexen-1-one + FMNH2 + O2 | 20% conversion | Pseudomonas putida | ? | - |
? | |
3-methyl-2-cyclopenten-1-one + FMNH2 + O2 | 10% conversion | Pseudomonas putida | ? | - |
? | |
4-phenyl-2-butanone + FMNH2 + O2 | 48% conversion | Pseudomonas putida | ? | - |
? | |
acetophenone + FMNH2 + O2 | 80% conversion | Pseudomonas putida | ? | - |
? | |
cyclobutanone + FMNH2 + O2 | 13% conversion | Pseudomonas putida | ? | - |
? | |
cyclohexanone + FMNH2 + O2 | 3% conversion | Pseudomonas putida | ? | - |
? | |
cyclopentanone + FMNH2 + O2 | 24% conversion | Pseudomonas putida | ? | - |
? | |
norcamphor + FMNH2 + O2 | 77% conversion | Pseudomonas putida | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
3,6-diketocamphane 1,6-monooxygenase | - |
Pseudomonas putida |
3,6-DKCMO | - |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | dependent on | Pseudomonas putida | |
additional information | dependent on | Pseudomonas putida |