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Literature summary for 1.14.14.154 extracted from

  • Alvarez-Rueda, N.; Fleury, A.; Morio, F.; Pagniez, F.; Gastinel, L.; Le Pape, P.
    Amino acid substitutions at the major insertion loop of Candida albicans sterol 14alpha-demethylase are involved in fluconazole resistance (2011), PLoS ONE, 6, e21239.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.154

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli TOP10F cells and in Pichia pastoris cells Candida albicans

Protein Variants

Protein Variants Comment Organism
K143R the mutant shows a sustained capacity for producing ergosterol, even in the presence of fluconazole Candida albicans
V456I the mutation leads to an 8fold increase in fluconazole minimal inhibitory concentrations of Pichia pastoris mutants compared to the wild type controls. The mutant shows a sustained capacity for producing ergosterol, even in the presence of fluconazole Candida albicans
Y447H the mutation leads to an 8fold increase in fluconazole minimal inhibitory concentrations of Pichia pastoris mutants compared to the wild type controls. The mutant shows a sustained capacity for producing ergosterol, even in the presence of fluconazole Candida albicans

Inhibitors

Inhibitors Comment Organism Structure
fluconazole
-
 Candida albicans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 x * 56000, SDS-PAGE Candida albicans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
lanosterol + [reduced NADPH-hemoprotein reductase] + O2 Candida albicans
-
 4,4-dimethylcholesta-8,14,24-trien-3-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Candida albicans
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lanosterol + [reduced NADPH-hemoprotein reductase] + O2
-
 Candida albicans 4,4-dimethylcholesta-8,14,24-trien-3-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 56000, SDS-PAGE Candida albicans

Synonyms

Synonyms Comment Organism
14alpha-demethylase
-
 Candida albicans
CYP51
-
 Candida albicans
Erg11p
-
 Candida albicans