Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.14.154 extracted from

  • Park, H.G.; Lee, I.S.; Chun, Y.J.; Yun, C.H.; Johnston, J.B.; Montellano, P.R.; Kim, D.
    Heterologous expression and characterization of the sterol 14alpha-demethylase CYP51F1 from Candida albicans (2011), Arch. Biochem. Biophys., 509, 9-15.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.154

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha cells Candida albicans

Protein Variants

Protein Variants Comment Organism
D116E the azole Kd value of the mutant enzyme is little altered compared to the wild type enzyme Candida albicans
F105L the azole Kd value of the mutant enzyme is little altered compared to the wild type enzyme Candida albicans
R467K the azole Kd value of the mutant enzyme is little altered compared to the wild type enzyme Candida albicans
Y132H the azole Kd value of the mutant enzyme is clearly altered compared to the wild type enzyme Candida albicans

Inhibitors

Inhibitors Comment Organism Structure
econazole
-
 Candida albicans
fluconazole
-
 Candida albicans
itraconazole
-
 Candida albicans
ketoconazole
-
 Candida albicans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61500
-
 x * 61500, SDS-PAGE Candida albicans

Organism

Organism UniProt Comment Textmining
Candida albicans P10613
-
-
Candida albicans ATCC MYA-2876 P10613
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, and gel filtration Candida albicans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lanosterol + [reduced NADPH-hemoprotein reductase] + O2
-
 Candida albicans 4,4-dimethylcholesta-8,14,24-trien-3-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
lanosterol + [reduced NADPH-hemoprotein reductase] + O2
-
 Candida albicans ATCC MYA-2876 4,4-dimethylcholesta-8,14,24-trien-3-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 61500, SDS-PAGE Candida albicans

Synonyms

Synonyms Comment Organism
CYP51F1
-
 Candida albicans
lanosterol 14alpha-demethylase
-
 Candida albicans

Cofactor

Cofactor Comment Organism Structure
heme
-
 Candida albicans