Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant MgCYP51 variants in a Saccharomyces cerevisiae mutant strain YUG37:erg11 carrying a doxycycline-regulatable tetO7-CYC promoter controlling native CYP51 expression. The wild-type MgCYP51 protein complements the function of the orthologous protein in the yeast, while mutants L50S, Y459D, and Y461H do not, only mutant I381V/Y459D/Y461H partially restores the activity of the yeast mutant | Zymoseptoria tritici |
Protein Variants | Comment | Organism |
---|---|---|
I381V | site-directed mutagenesis, the mutant enzyme does not complement the CYP-deficient Saccharomyces cerevisiae mutant strain YUG37:erg11 | Zymoseptoria tritici |
I381V/Y459D/Y461H | site-directed mutagenesis, the mutant enzyme does partially complement the CYP-deficient Saccharomyces cerevisiae mutant strain YUG37:erg11 | Zymoseptoria tritici |
L50S | site-directed mutagenesis, the mutant enzyme does not complement the CYP-deficient Saccharomyces cerevisiae mutant strain YUG37:erg11 | Zymoseptoria tritici |
additional information | construction of the deletion mutant DELTAY459/G460 | Zymoseptoria tritici |
Y459D | site-directed mutagenesis, the mutant enzyme does not complement the CYP-deficient Saccharomyces cerevisiae mutant strain YUG37:erg11 | Zymoseptoria tritici |
Y461H | site-directed mutagenesis, the mutant enzyme does not complement the CYP-deficient Saccharomyces cerevisiae mutant strain YUG37:erg11 | Zymoseptoria tritici |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Zymoseptoria tritici | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zymoseptoria tritici | - |
gene CYP51 | - |
Zymoseptoria tritici IPO323 | - |
gene CYP51 | - |
Synonyms | Comment | Organism |
---|---|---|
CYP51 | - |
Zymoseptoria tritici |
eburicol 14alpha-demethylase | - |
Zymoseptoria tritici |