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Literature summary for 1.14.14.154 extracted from
- Expression of microsomal lanosterol 14alpha-demethylase (CYP51) in an engineered soluble monomeric form (2008), Biochem. Biophys. Res. Commun., 371, 855-859.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of CYP51 mutant soluble, monomeric enzymes in Escherichia coli | Bos taurus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of a soluble, monomeric enzyme, two variants of bovine CYP51 with different truncations and modifications in their Nterminal membrane-spanning domains, overview | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ketoconazole | - |
Bos taurus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum membrane | - |
Bos taurus | 5789 | - |
| microsome | - |
Bos taurus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | a cytochrome P450 enzyme | Bos taurus | |
| Mg2+ | - |
Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
CYP51 mutant variant 1, gel filtration | Bos taurus |
| 50000 | - |
CYP51 mutant variant 2, gel filtration | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Bos taurus | - |
4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | Q4PJW3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant soluble, monomeric CYP51 truncation mutants from Escherichia coli in a procedure comprising several steps, overview | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00756 | - |
purified recombinant truncated, soluble, monomeric enzyme | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Bos taurus | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 44000, CYP51 mutant variant 1, 2 * 50000, CYP mutant variant 2 | Bos taurus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CYP51 | - |
Bos taurus |
| lanosterol 14alpha-demethylase | - |
Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome P450 | a cytochrome P450 enzyme | Bos taurus |
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Release 2023.1 (January 2023)
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