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Literature summary for 1.14.14.15 extracted from

  • Lin, S.; Van Lanen, S.G.; Shen, B.
    Characterization of the two-component, FAD-dependent monooxygenase SgcC that requires carrier protein-tethered substrates for the biosynthesis of the enediyne antitumor antibiotic C-1027 (2008), J. Am. Chem. Soc., 130, 6616-6623.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overproduced as an N-terminal His6-tagged fusion protein Streptomyces globisporus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.742
-
S-((3S)-3-amino-3-(3-chloro-4-hydroxyphenyl)propanoyl)-[peptidyl-carrier protein SgcC2] pH 6.0, 25°C Streptomyces globisporus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63200
-
x * 63200, calculated from sequence, SDS-PAGE Streptomyces globisporus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-((3S)-3-amino-3-(3-chloro-4-hydroxyphenyl)propanoyl)-[peptidyl-carrier protein SgcC2] + FADH2 + O2 Streptomyces globisporus the enzyme is involved in the biosynthesis of the (S)-3-chloro-5-hydroxy-beta-tyrosine moiety prior to incorporation into the chromoprotein antitumor antibiotic C-1027. FADH2 is supplied by the C-1027 pathway-specific flavin reductase SgcE6 S-((3S)-3-amino-3-(3-chloro-4,5-dihydroxyphenyl)propanoyl)-[peptidyl-carrier protein SgcC2] + FAD + H2O
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces globisporus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces globisporus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with [peptidyl-carrier-protein SgcC2]-beta-tyrosyl thioester and [peptidyl-carrier-protein SgcC2]-(S)-3-hydroxy-beta-tyrosyl thioester Streptomyces globisporus ?
-
?
S-((3S)-3-amino-3-(3-bromo-4-hydroxyphenyl)propanoyl)-[peptidyl-carrier protein SgcC2] + FADH2 + O2
-
Streptomyces globisporus S-((3S)-3-amino-3-(3-bromo-4,5-dihydroxyphenyl)propanoyl)-[peptidyl-carrier protein SgcC2] + FAD + H2O
-
?
S-((3S)-3-amino-3-(3-chloro-4-hydroxyphenyl)propanoyl)-[peptidyl-carrier protein SgcC2] + FADH2 + O2
-
Streptomyces globisporus S-((3S)-3-amino-3-(3-chloro-4,5-dihydroxyphenyl)propanoyl)-[peptidyl-carrier protein SgcC2] + FAD + H2O
-
?
S-((3S)-3-amino-3-(3-chloro-4-hydroxyphenyl)propanoyl)-[peptidyl-carrier protein SgcC2] + FADH2 + O2 the enzyme is involved in the biosynthesis of the (S)-3-chloro-5-hydroxy-beta-tyrosine moiety prior to incorporation into the chromoprotein antitumor antibiotic C-1027. FADH2 is supplied by the C-1027 pathway-specific flavin reductase SgcE6 Streptomyces globisporus S-((3S)-3-amino-3-(3-chloro-4,5-dihydroxyphenyl)propanoyl)-[peptidyl-carrier protein SgcC2] + FAD + H2O
-
?
S-((3S)-3-amino-3-(3-fluoro-4-hydroxyphenyl)propanoyl)-[peptidyl-carrier protein SgcC2] + FADH2 + O2
-
Streptomyces globisporus S-((3S)-3-amino-3-(3-fluoro-4,5-dihydroxyphenyl)propanoyl)-[peptidyl-carrier protein SgcC2] + FAD + H2O
-
?
S-((3S)-3-amino-3-(3-iodo-4-hydroxyphenyl)propanoyl)-[peptidyl-carrier protein SgcC2] + FADH2 + O2
-
Streptomyces globisporus S-((3S)-3-amino-3-(3-iodo-4,5-dihydroxyphenyl)propanoyl)-[peptidyl-carrier protein SgcC2] + FAD + H2O
-
?
S-((3S)-3-amino-3-(3-methyl-4-hydroxyphenyl)propanoyl)-[peptidyl-carrier protein SgcC2] + FADH2 + O2
-
Streptomyces globisporus S-((3S)-3-amino-3-(3-methyl-4,5-dihydroxyphenyl)propanoyl)-[peptidyl-carrier protein SgcC2] + FAD + H2O
-
?

Subunits

Subunits Comment Organism
? x * 63200, calculated from sequence, SDS-PAGE Streptomyces globisporus

Synonyms

Synonyms Comment Organism
SgcC
-
Streptomyces globisporus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Streptomyces globisporus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.023
-
S-((3S)-3-amino-3-(3-chloro-4-hydroxyphenyl)propanoyl)-[peptidyl-carrier protein SgcC2] pH 6.0, 25°C Streptomyces globisporus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Streptomyces globisporus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7 pH 5.5: about 50% of maximal activity, pH 7.0: about 50% of maximal activity Streptomyces globisporus

General Information

General Information Comment Organism
physiological function the enzyme is involved in the biosynthesis of the (S)-3-chloro-5-hydroxy-beta-tyrosine moiety prior to incorporation into the chromoprotein antitumor antibiotic C-1027 Streptomyces globisporus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.031
-
S-((3S)-3-amino-3-(3-chloro-4-hydroxyphenyl)propanoyl)-[peptidyl-carrier protein SgcC2] pH 6.0, 25°C Streptomyces globisporus