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Literature summary for 1.14.14.148 extracted from

  • Larbat, R.; Hehn, A.; Hans, J.; Schneider, S.; Jugde, H.; Schneider, B.; Matern, U.; Bourgaud, F.
    Isolation and functional characterization of CYP71AJ4 encoding for the first P450 monooxygenase of angular furanocoumarin biosynthesis (2009), J. Biol. Chem., 284, 4776-4785.
    View publication on PubMed
Search for more literature for 1.14.14.148

Cloned(Commentary)

Cloned (Comment) Organism
expressed in yeast Pastinaca sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0014
-
 syn-[3'D]columbianetin pH 7.0, formation of angelicin Pastinaca sativa
0.0014
-
 (+)-columbianetin pH 7.0, isopropyloxy-hydroxylation of substrate Pastinaca sativa
0.002
-
 syn-[3'D]columbianetin pH 7.0, isopropyloxy-hydroxylation of substrate Pastinaca sativa
0.0021
-
 (+)-columbianetin pH 7.0, formation of angelicin Pastinaca sativa
0.0038
-
 syn-[3'D]columbianetin pH 7.0, anti-3'-hydroxylation of substrate Pastinaca sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-columbianetin + [reduced NADPH-hemoprotein reductase] + O2 Pastinaca sativa the enzyme is involved in angular furanocoumarin pathway angelicin + acetone + [oxidized NADPH-hemoprotein reductase] + H2O negligible amounts of a hydroxylated columbianetin by-product ?

Organism

Organism UniProt Comment Textmining
Pastinaca sativa C0SJS3 fragment
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Pastinaca sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-columbianetin + [reduced NADPH-hemoprotein reductase] + O2 the enzyme is involved in angular furanocoumarin pathway Pastinaca sativa angelicin + acetone + [oxidized NADPH-hemoprotein reductase] + H2O negligible amounts of a hydroxylated columbianetin by-product ?
(+)-columbianetin + [reduced NADPH-hemoprotein reductase] + O2 angelicin synthase attacks columbianetin by syn-elimination of hydrogen from C-3' Pastinaca sativa angelicin + acetone + [oxidized NADPH-hemoprotein reductase] + H2O negligible amounts of a hydroxylated columbianetin by-product ?
syn-[3'D]columbianetin + [reduced NADPH-hemoprotein reductase] + O2
-
 Pastinaca sativa syn-[3'D](anti-3'-hydroxy)-columbianetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

Synonyms Comment Organism
angelicin synthase monooxygenase involved in angular furanocoumarin biosynthesis Pastinaca sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
27 30
-
 Pastinaca sativa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.06
-
 syn-[3'D]columbianetin pH 7.0, isopropyloxy-hydroxylation of substrate Pastinaca sativa
0.27
-
 syn-[3'D]columbianetin pH 7.0, formation of angelicin Pastinaca sativa
0.32
-
 (+)-columbianetin pH 7.0, isopropyloxy-hydroxylation of substrate Pastinaca sativa
1.9
-
 (+)-columbianetin pH 7.0, formation of angelicin Pastinaca sativa
3.9
-
 syn-[3'D]columbianetin pH 7.0, anti-3'-hydroxylation of substrate Pastinaca sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Pastinaca sativa

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
-
 Pastinaca sativa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
30
-
 syn-[3'D]columbianetin pH 7.0, isopropyloxy-hydroxylation of substrate Pastinaca sativa
188
-
 syn-[3'D]columbianetin pH 7.0, formation of angelicin Pastinaca sativa
227
-
 (+)-columbianetin pH 7.0, isopropyloxy-hydroxylation of substrate Pastinaca sativa
920
-
 (+)-columbianetin pH 7.0, formation of angelicin Pastinaca sativa
967
-
 syn-[3'D]columbianetin pH 7.0, anti-3'-hydroxylation of substrate Pastinaca sativa