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Literature summary for 1.14.14.142 extracted from
- 8-dimethylallylnaringenin 2'-hydroxylase, the crucial cytochrome P450 mono-oxygenase for lavandulylated flavanone formation in Sophora flavescens cultured cells (2001), Phytochemistry, 58, 671-676.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| carbon monoxide | - |
Sophora flavescens |  |
| cytochrome c | 70% inhibition at 10 mM | Sophora flavescens | |
| Metyrapone | 34% inhibition at 1 mM | Sophora flavescens | |
| miconazole | 36% inhibition at 1 mM | Sophora flavescens | |
| additional information | no or poor inhibition by ancymidol and KCN | Sophora flavescens | |
| tropolon | 30% inhibition at 1 mM | Sophora flavescens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.055 | - |
8-dimethylallylnaringenin | - |
Sophora flavescens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | the enzyme is tightly bound to the membrane | Sophora flavescens | 16020 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 8-dimethylallylnaringenin + [reduced NADPH-hemoprotein reductase] + O2 | Sophora flavescens | the enzyme is crucial for lavandulylated flavanone formation, the enzyme catalyzes the 2'-hydroxylation of 8-DMAN, which is then formed to sophoraflavanone G, pathway overview | leachianone G + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sophora flavescens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Sophora flavescens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (-)-(2S)-8-dimethylallylnaringenin + [oxidized NADPH-hemoprotein reductase] + H2O | i.e. sophoraflavanone B, the enzyme catalyzes the 2'-hydroxylation of 8-DMAN and is O2-dependent | Sophora flavescens | leachianone G + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 8-dimethylallylnaringenin + [reduced NADPH-hemoprotein reductase] + O2 | the enzyme is crucial for lavandulylated flavanone formation, the enzyme catalyzes the 2'-hydroxylation of 8-DMAN, which is then formed to sophoraflavanone G, pathway overview | Sophora flavescens | leachianone G + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| additional information | the enzyme is a cytochrome P450 monooxygenase | Sophora flavescens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 8-DMAN 2'-hydroxylase | - |
Sophora flavescens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Sophora flavescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Sophora flavescens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme is a cytochrome P450 monooxygenase | Sophora flavescens |
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