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Literature summary for 1.14.14.139 extracted from

  • Murakami, K.; Okada, Y.; Okuda, K.
    Purification and characterization of 7alpha-hydroxy-4-cholesten-3-one 12alpha-monooxygenase (1982), J. Biol. Chem., 257, 8030-8035.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information NADPH-cytochrome P-450 reductase, EC 1.6.2.4, is required for maximal activity Oryctolagus cuniculus

General Stability

General Stability Organism
stabilized at least 4 weeks under high buffer concentrations such as 300 mM phosphate buffer Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
5beta-cholestane-3alpha,7alpha,12alpha-triol 0.066 mM, 62% inhibition Oryctolagus cuniculus
5beta-cholestane-3alpha,7alpha-diol 0.066 mM, 30% inhibition Oryctolagus cuniculus
Emulgen 913 0.1% w/v, complete inhibition Oryctolagus cuniculus
MgCl2 1 mM, 10% inhibition Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.027
-
7alpha-hydroxycholest-4-en-3-one
-
Oryctolagus cuniculus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Oryctolagus cuniculus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
non-denaturing PAGE Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Oryctolagus cuniculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7alpha-hydroxycholest-4-en-3-one + NADPH + H+ + O2
-
Oryctolagus cuniculus 7alpha,12alpha-dihydroxycholest-4-en-3-one + NADP+ + H2O
-
?

Synonyms

Synonyms Comment Organism
7alpha-hydroxy-4-cholesten-3-one 12alpha-monooxygenase
-
Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7 7
-
Oryctolagus cuniculus

pH Range

pH Minimum pH Maximum Comment Organism
6 7.5 pH 6.0: about 40% of maximal activity, pH 7.5: about 65% of maximal activity Oryctolagus cuniculus

Cofactor

Cofactor Comment Organism Structure
cytochrome P-450
-
Oryctolagus cuniculus
NADPH
-
Oryctolagus cuniculus