Cloned (Comment) | Organism |
---|---|
gene CYP707A1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, real-time quantitative RT-PCR enzyme expression analysis. Functional expression of AhCYP707A1 cDNA in yeast with recombinant AhCYP707A1 ABA 8-hydroxylase catalytic activity in the microsomal fractions | Arachis hypogaea |
gene CYP707A2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, real-time quantitative RT-PCR enzyme expression analysis | Arachis hypogaea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Arachis hypogaea | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-abscisate + [reduced NADPH-hemoprotein reductase] + O2 | Arachis hypogaea | - |
8'-hydroxyabscisate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arachis hypogaea | U6NHS3 | cv. Yueyou 7 | - |
Arachis hypogaea | U6NJF1 | cv. Yueyou 7 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arachis hypogaea | - |
additional information | isozymes AhCYP707A1 and AhCYP707A2 are expressed ubiquitously in peanut roots, stems, and leaves with different transcript accumulation levels, including the higher expression of AhCYP707A1 in roots | Arachis hypogaea | - |
root | - |
Arachis hypogaea | - |
root | high expression | Arachis hypogaea | - |
seedling | - |
Arachis hypogaea | - |
stem | - |
Arachis hypogaea | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-abscisate + [reduced NADPH-hemoprotein reductase] + O2 | - |
Arachis hypogaea | 8'-hydroxyabscisate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 53390, sequence calculation | Arachis hypogaea |
? | x * 55060, sequence calculation | Arachis hypogaea |
Synonyms | Comment | Organism |
---|---|---|
ABA 8'-hydroxylase | - |
Arachis hypogaea |
AhCYP707A1 | - |
Arachis hypogaea |
AhCYP707A2 | - |
Arachis hypogaea |
CYP707A1 | - |
Arachis hypogaea |
CYP707A2 | - |
Arachis hypogaea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Arachis hypogaea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Arachis hypogaea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P-450 | - |
Arachis hypogaea | |
NADPH-hemoprotein reductase | A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ | Arachis hypogaea |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Arachis hypogaea | sequence calculation | - |
9.16 |
Arachis hypogaea | sequence calculation | - |
9.22 |
Organism | Comment | Expression |
---|---|---|
Arachis hypogaea | susceptibility to osmotic stress and the resistance to salt ions in peanut seedlings of CYP707A isozymes. The osmotic stress instead of ionic stress affects the expression of those genes and the biosynthesis of abscisic acid in peanut. No effect by Li+ | additional information |
Arachis hypogaea | expression of AhCYP707A2 is significantly up-regulated by 20% PEG 6000 or 250 mmol/l NaCl in peanut roots, stems, and leaves, whereas the up-regulation of AhCYP707A1 transcript level by PEG 6000 or NaCl is observed only in roots instead of leaves and stems | up |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the cytochrome P450 CYP707A family. The expressions of isozymes AhCYP707A1 and AhCYP707A2 play an important role in abscisic acid catabolism in peanut, particularly in response to osmotic stress | Arachis hypogaea |
physiological function | abscisic acid (ABA) catabolism is one of the determinants of endogenous ABA levels affecting numerous aspects of plant growth and abiotic-stress responses. The major ABA catabolic pathway is triggered by ABA 8-hydroxylation catalysed by ABA 8'-hydroxylase | Arachis hypogaea |