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Literature summary for 1.14.14.133 extracted from

  • Slessor, K.E.; Farlow, A.J.; Cavaignac, S.M.; Stok, J.E.; De Voss, J.J.
    Oxygen activation by P450(cin): Protein and substrate mutagenesis (2011), Arch. Biochem. Biophys., 507, 154-162.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
N242T significant drop in the rate of NADPH consumption. In addition to wild-type product (1R)-6beta-hydroxycineole, products (1R)-6alpha-hydroxycineole 2b and (1S)-6alpha-hydroxycineole are formed at 22% and 31%, respectively Citrobacter braakii
N242T/T243A significant drop in the rate of NADPH consumption. In addition to wild-type product (1R)-6beta-hydroxycineole, products (1R)-6alpha-hydroxycineole 2b and (1S)-6alpha-hydroxycineole are formed at 18% and 39%, respectively Citrobacter braakii
T243A increase in the rate of NADPH consumption of 30%. Like in wild-type, single product is (1R)-6beta-hydroxycineole. T243 is not involved in controlling the protonation of the hydroperoxy species Citrobacter braakii

Organism

Organism UniProt Comment Textmining
Citrobacter braakii Q8VQF6
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,8-cineole + [reduced flavodoxin] + O2
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Citrobacter braakii (1R)-6beta-hydroxycineole + [oxidized flavodoxin] + H2O single product ?
2,2-dimethylbicyclo[2.2.2]octane + [reduced flavodoxin] + O2 i.e. cinane Citrobacter braakii ? + [oxidized flavodoxin] + H2O identification of least seven oxidised derivatives ?
camphane + [reduced flavodoxin] + O2
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Citrobacter braakii camphor + [oxidized flavodoxin] + H2O main product in presence of excess NADPH, plus epi-camphor at a rate of 3:1, and several minor products ?
additional information a hydroxyl group on the substrate is vital, and in its absence catalytic turnover is effectively abolished. In the absence of the ethereal oxygen there is still a significant amount of coupling of the NADPH-reducing equivalents to the formation of oxidised product. The substrate itself is not important in controlling oxygen activation, but is essential for regio- and stereoselective substrate oxidation Citrobacter braakii ?
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