Protein Variants | Comment | Organism |
---|---|---|
N242T | significant drop in the rate of NADPH consumption. In addition to wild-type product (1R)-6beta-hydroxycineole, products (1R)-6alpha-hydroxycineole 2b and (1S)-6alpha-hydroxycineole are formed at 22% and 31%, respectively | Citrobacter braakii |
N242T/T243A | significant drop in the rate of NADPH consumption. In addition to wild-type product (1R)-6beta-hydroxycineole, products (1R)-6alpha-hydroxycineole 2b and (1S)-6alpha-hydroxycineole are formed at 18% and 39%, respectively | Citrobacter braakii |
T243A | increase in the rate of NADPH consumption of 30%. Like in wild-type, single product is (1R)-6beta-hydroxycineole. T243 is not involved in controlling the protonation of the hydroperoxy species | Citrobacter braakii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrobacter braakii | Q8VQF6 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,8-cineole + [reduced flavodoxin] + O2 | - |
Citrobacter braakii | (1R)-6beta-hydroxycineole + [oxidized flavodoxin] + H2O | single product | ? | |
2,2-dimethylbicyclo[2.2.2]octane + [reduced flavodoxin] + O2 | i.e. cinane | Citrobacter braakii | ? + [oxidized flavodoxin] + H2O | identification of least seven oxidised derivatives | ? | |
camphane + [reduced flavodoxin] + O2 | - |
Citrobacter braakii | camphor + [oxidized flavodoxin] + H2O | main product in presence of excess NADPH, plus epi-camphor at a rate of 3:1, and several minor products | ? | |
additional information | a hydroxyl group on the substrate is vital, and in its absence catalytic turnover is effectively abolished. In the absence of the ethereal oxygen there is still a significant amount of coupling of the NADPH-reducing equivalents to the formation of oxidised product. The substrate itself is not important in controlling oxygen activation, but is essential for regio- and stereoselective substrate oxidation | Citrobacter braakii | ? | - |
? |