Literature summary for 1.14.14.121 extracted from

Wang, L.; Zhao, S.J.; Liang, Y.L.; Sun, Y.; Cao, H.J.; Han, Y.
Identification of the protopanaxatriol synthase gene CYP6H for ginsenoside biosynthesis in Panax quinquefolius (2014), Funct. Integr. Genomics, 14, 559-570 .

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Cloned(Commentary)

Cloned (Comment)	Organism
gene CYP6H, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, RT-PCR expression analysis, functional recombinant ectopic expression of gene CYP6H in Saccharomyces cerevisiae results in the production of protopanaxatriol with added exogenous protopanaxadiol, mass spectrometric analysis, recombinant overexpression in Panax quinquefolius hairy roots from pBI121-CYP6H-overexpression plasmid	Panax quinquefolius

Cloned (Comment)

Organism

gene CYP6H, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, RT-PCR expression analysis, functional recombinant ectopic expression of gene CYP6H in Saccharomyces cerevisiae results in the production of protopanaxatriol with added exogenous protopanaxadiol, mass spectrometric analysis, recombinant overexpression in Panax quinquefolius hairy roots from pBI121-CYP6H-overexpression plasmid

Panax quinquefolius

Protein Variants

Protein Variants	Comment	Organism
additional information	enzyme silencing by expression of RNAi in hairy roots of Panax quinquefolius	Panax quinquefolius

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
protopanaxadiol + [reduced NADPH-hemoprotein reductase] + O2	Panax quinquefolius	-	protopanaxatriol + [oxidized NADPH-hemoprotein reductase] + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Panax quinquefolius	L7TF20	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
hairy root	-	Panax quinquefolius	-
root	-	Panax quinquefolius	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
protopanaxadiol + [reduced NADPH-hemoprotein reductase] + O2	-	Panax quinquefolius	protopanaxatriol + [oxidized NADPH-hemoprotein reductase] + H2O	-	?

Synonyms

Synonyms	Comment	Organism
CYP6H	-	Panax quinquefolius
protopanaxatriol synthase	-	Panax quinquefolius

Cofactor

Cofactor	Comment	Organism	Structure
cytochrome P450	-	Panax quinquefolius
NADPH-hemoprotein reductase	A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._	Panax quinquefolius

Expression

Organism	Comment	Expression
Panax quinquefolius	the gene CYP6H shows no obvious transcription change in methyl jasmonate (MeJA)-induced hairy roots	additional information

General Information

General Information	Comment	Organism
metabolism	protopanaxadiol 6-hydroxylase converts protopanaxadiol into protopanaxatriol in Panax quinquefolius ginsenoside biosynthesis, pathway overview	Panax quinquefolius
physiological function	protopanaxatriol synthase, which is considered cytochrome P450 (CYP450) in ginsenoside biosynthesis pathway can convert protopanaxadiol into protopanaxatriol	Panax quinquefolius