Literature summary for 1.14.14.120 extracted from

Wang, L.; Zhao, S.J.; Cao, H.J.; Sun, Y.
The isolation and characterization of dammarenediol synthase gene from Panax quinquefolius and its heterologous co-expression with cytochrome P450 gene PqD12H in yeast (2014), Funct. Integr. Genomics, 14, 545-557 .

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Cloned(Commentary)

Cloned (Comment)	Organism
gene PqD12H, expression of PqD12H in Saccharmoyces cerevisiae strain WAT21 from plasmid pAUR123-PqD12H, RT-PCR enzyme expression analysis, recombinant coexpression with dammarenediol synthase DS leads to increased accumulation of ginsenosides, i.e. protopanaxatriol protopanaxadiol. compared to control, both PqDS and PqD12H have higher accumulation of mRNA in the transgenic hairy roots and the increase ratio decreased slightly over culture time. Yeast with single recombinant vector pAUR123-PqD12H is unable to express protopanaxadiol without exogenous dammarenediol-II	Panax quinquefolius

Cloned (Comment)

Organism

gene PqD12H, expression of PqD12H in Saccharmoyces cerevisiae strain WAT21 from plasmid pAUR123-PqD12H, RT-PCR enzyme expression analysis, recombinant coexpression with dammarenediol synthase DS leads to increased accumulation of ginsenosides, i.e. protopanaxatriol protopanaxadiol. compared to control, both PqDS and PqD12H have higher accumulation of mRNA in the transgenic hairy roots and the increase ratio decreased slightly over culture time. Yeast with single recombinant vector pAUR123-PqD12H is unable to express protopanaxadiol without exogenous dammarenediol-II

Panax quinquefolius

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
dammarenediol-II + [reduced NADPH-hemoprotein reductase] + O2	Panax quinquefolius	-	protopanaxadiol + [oxidized NADPH-hemoprotein reductase] + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Panax quinquefolius	K4K542	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
hairy root	-	Panax quinquefolius	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dammarenediol-II + [reduced NADPH-hemoprotein reductase] + O2	-	Panax quinquefolius	protopanaxadiol + [oxidized NADPH-hemoprotein reductase] + H2O	-	?

Synonyms

Synonyms	Comment	Organism
PqD12H	-	Panax quinquefolius
protopanaxadiol synthase	-	Panax quinquefolius

Cofactor

Cofactor	Comment	Organism	Structure
cytochrome P450	-	Panax quinquefolius
NADPH-hemoprotein reductase	A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._	Panax quinquefolius