Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.14.117 extracted from

  • Yu, J.; Chang, P.K.; Ehrlich, K.C.; Cary, J.W.; Montalbano, B.; Dyer, J.M.; Bhatnagar, D.; Cleveland, T.E.
    Characterization of the critical amino acids of an Aspergillus parasiticus cytochrome P-450 monooxygenase encoded by ordA that is involved in the biosynthesis of aflatoxins B1, G1, B2, and G2 (1998), Appl. Environ. Microbiol., 64, 4834-4841.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.117

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae strain InvSc1 Aspergillus parasiticus

Protein Variants

Protein Variants Comment Organism
A143S the mutation affects enzymatic activity (approximately 50% of the enzyme activity which converts O-methylsterigmatocystin to aflatoxin B1 is lost) Aspergillus parasiticus
H400L the mutation results in a loss of the monooxygenase activity Aspergillus parasiticus
I528Y the mutation does not affect enzymatic activity Aspergillus parasiticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2 Aspergillus parasiticus
-
 aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2 Aspergillus parasiticus SRRC 143
-
 aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2 Aspergillus parasiticus
-
 aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
 ?
8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2 Aspergillus parasiticus SRRC 143
-
 aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus parasiticus O13345
-
-
Aspergillus parasiticus SRRC 143 O13345
-
-

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Aspergillus parasiticus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus parasiticus aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus parasiticus SRRC 143 aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus parasiticus aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
 ?
8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus parasiticus SRRC 143 aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2
-
 ?

Synonyms

Synonyms Comment Organism
ordA
-
 Aspergillus parasiticus

General Information

General Information Comment Organism
metabolism the enzyme is involved in the biosynthesis of aflatoxins B1, G1, B2, and G2 Aspergillus parasiticus