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Literature summary for 1.14.14.115 extracted from

  • Seki, H.; Sawai, S.; Ohyama, K.; Mizutani, M.; Ohnishi, T.; Sudo, H.; Fukushima, E.O.; Akashi, T.; Aoki, T.; Saito, K.; Muranaka, T.
    Triterpene functional genomics in Licorice for identification of CYP72A154 involved in the biosynthesis of glycyrrhizin (2011), Plant Cell, 23, 4112-4123.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.115

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH10Bac cells and in Saccharomyces cerevisiae strain BJ2168 Glycyrrhiza uralensis
expressed in Saccharomyces cerevisiae strain BJ2168 that endogenously produces 11-oxo-beta-amyrin Glycyrrhiza uralensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
11-oxo-beta-amyrin + O2 + [reduced NADPH-hemoprotein reductase] Glycyrrhiza uralensis the enzyme mainly catalyzes the C-30 hydroxylation of 11-oxo-beta-amyrin to yield 30-hydroxy-11-oxo-beta-amyrin as the major product, accompanied by its isomers as minor products 30-hydroxy-11-oxo-beta-amyrin + [oxidized NADPH-hemoprotein reductase] + H2O
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 ?

Organism

Organism UniProt Comment Textmining
Glycyrrhiza uralensis
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-
-

Source Tissue

Source Tissue Comment Organism Textmining
colon
-
 Glycyrrhiza uralensis
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additional information not detected in leaves Glycyrrhiza uralensis
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root
-
 Glycyrrhiza uralensis
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stem
-
 Glycyrrhiza uralensis
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stolon
-
 Glycyrrhiza uralensis
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
11-oxo-beta-amyrin + O2 + [reduced NADPH-hemoprotein reductase] single oxidation reaction catalyzed by CYP72A154 Glycyrrhiza uralensis 30-hydroxy-11-oxo-beta-amyrin + [oxidized NADPH-hemoprotein reductase] + H2O
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 ?
11-oxo-beta-amyrin + O2 + [reduced NADPH-hemoprotein reductase] the enzyme mainly catalyzes the C-30 hydroxylation of 11-oxo-beta-amyrin to yield 30-hydroxy-11-oxo-beta-amyrin as the major product, accompanied by its isomers as minor products Glycyrrhiza uralensis 30-hydroxy-11-oxo-beta-amyrin + [oxidized NADPH-hemoprotein reductase] + H2O
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 ?
30-hydroxy-11-oxo-beta-amyrin + O2 + [reduced NADPH-hemoprotein reductase]
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 Glycyrrhiza uralensis glycyrrhetaldehyde + [oxidized NADPH-hemoprotein reductase] + 2 H2O
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 ?
30-hydroxy-11-oxo-beta-amyrin + O2 + [reduced NADPH-hemoprotein reductase] single oxidation reaction catalyzed by CYP72A154 Glycyrrhiza uralensis glycyrrhetaldehyde + [oxidized NADPH-hemoprotein reductase] + 2 H2O
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 ?
glycyrrhetaldehyde + O2 + [reduced NADPH-hemoprotein reductase]
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 Glycyrrhiza uralensis glycyrrhetinate + [oxidized NADPH-hemoprotein reductase] + H2O
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 ?

Synonyms

Synonyms Comment Organism
CYP72A154
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 Glycyrrhiza uralensis
CYP88D6
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 Glycyrrhiza uralensis

General Information

General Information Comment Organism
metabolism the beta-amyrin 11-oxidase gene CYP88D6 is the initial P450 gene in glycyrrhizin biosynthesis. CYP72A154 is responsible for C-30 oxidation of beta-amyrin in the glycyrrhizin pathway Glycyrrhiza uralensis
metabolism the enzyme is involved in the biosynthesis of glycyrrhizin Glycyrrhiza uralensis