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Literature summary for 1.14.14.110 extracted from

  • Bailey, B.A.; Larson, R.L.
    Maize microsomal benzoxazinone N-monooxygenase (1991), Plant Physiol., 95, 792-796.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
EDTA 49% enhancement of activity at 5 mM Zea mays
additional information not affected by 5 mM diethyldithiocarbamate Zea mays

General Stability

General Stability Organism
extraction of microsomes in the absence of dithioerythritol results in total loss of activity, but exclusion of dithioerythritol from the assay buffer has no effect on enzyme activity Zea mays

Inhibitors

Inhibitors Comment Organism Structure
2,2'-dipyridyl 5 mM inhibits activity by 57% Zea mays
Ca2+ 5 mM Ca2+ inhibits activity by 69% Zea mays
carbon monoxide the effect of carbon monoxide can be reversed by exposing the enzyme to 450 nanometer light during the incubation period Zea mays
cytochrome c
-
Zea mays
Fe2+ 5 mM Fe2+ inhibits activity by 64% Zea mays
Hg2+ complete inhibition at 5 mM Zea mays
Mg2+ 5 mM Mg2+ inhibits activity by 45% Zea mays
Mn2+ complete inhibition at 5 mM Zea mays
N-ethylmaleimide 5 mM inhibits activity by 97% Zea mays
N2 the effect of nitrogen can be reversed by exposing the enzyme to air Zea mays
NADP+
-
Zea mays
NaN3 5 mM NaN3 inhibits activity by 16% Zea mays
p-chloromercuribenzoate 5 mM inhibits activity by 58% Zea mays
Zn2+ complete inhibition at 5 mM Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
NADPH in 50 mM HEPES, pH 8.5, at 35°C Zea mays
0.013
-
2-hydroxy-2H-1,4-benzoxazin-3(4H)-one in 50 mM HEPES, pH 8.5, at 35°C Zea mays

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Zea mays
-
-

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
Zea mays
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxy-2H-1,4-benzoxazin-3(4H)-one + NADPH + H+ + O2
-
Zea mays 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one + NADP+ + H2O
-
r
2-hydroxy-2H-1,4-benzoxazin-3(4H)-one + [reduced NADPH-hemoprotein reductase] + O2
-
Zea mays 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
additional information no activity with NADH and FAD Zea mays ?
-
?

Synonyms

Synonyms Comment Organism
benzoxazinone N-monooxygenase
-
Zea mays

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Zea mays

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
heating the enzyme for 10 min at 100°C in a boiling water bath results in a total loss of activity Zea mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Zea mays

Cofactor

Cofactor Comment Organism Structure
cytochrome P-450 dependent on Zea mays