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Literature summary for 1.14.14.110 extracted from
- Maize microsomal benzoxazinone N-monooxygenase (1991), Plant Physiol., 95, 792-796.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 49% enhancement of activity at 5 mM | Zea mays |  |
| additional information | not affected by 5 mM diethyldithiocarbamate | Zea mays |
General Stability
| General Stability | Organism |
|---|---|
| extraction of microsomes in the absence of dithioerythritol results in total loss of activity, but exclusion of dithioerythritol from the assay buffer has no effect on enzyme activity | Zea mays |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,2'-dipyridyl | 5 mM inhibits activity by 57% | Zea mays | |
| Ca2+ | 5 mM Ca2+ inhibits activity by 69% | Zea mays | |
| carbon monoxide | the effect of carbon monoxide can be reversed by exposing the enzyme to 450 nanometer light during the incubation period | Zea mays | |
| cytochrome c | - |
Zea mays | |
| Fe2+ | 5 mM Fe2+ inhibits activity by 64% | Zea mays | |
| Hg2+ | complete inhibition at 5 mM | Zea mays | |
| Mg2+ | 5 mM Mg2+ inhibits activity by 45% | Zea mays | |
| Mn2+ | complete inhibition at 5 mM | Zea mays | |
| N-ethylmaleimide | 5 mM inhibits activity by 97% | Zea mays | |
| N2 | the effect of nitrogen can be reversed by exposing the enzyme to air | Zea mays | |
| NADP+ | - |
Zea mays | |
| NaN3 | 5 mM NaN3 inhibits activity by 16% | Zea mays | |
| p-chloromercuribenzoate | 5 mM inhibits activity by 58% | Zea mays | |
| Zn2+ | complete inhibition at 5 mM | Zea mays | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | - |
NADPH | in 50 mM HEPES, pH 8.5, at 35°C | Zea mays | |
| 0.013 | - |
2-hydroxy-2H-1,4-benzoxazin-3(4H)-one | in 50 mM HEPES, pH 8.5, at 35°C | Zea mays | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Zea mays | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Zea mays | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-hydroxy-2H-1,4-benzoxazin-3(4H)-one + NADPH + H+ + O2 | - |
Zea mays | 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one + NADP+ + H2O | - |
r | |
| 2-hydroxy-2H-1,4-benzoxazin-3(4H)-one + [reduced NADPH-hemoprotein reductase] + O2 | - |
Zea mays | 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| additional information | no activity with NADH and FAD | Zea mays | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| benzoxazinone N-monooxygenase | - |
Zea mays |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Zea mays |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
heating the enzyme for 10 min at 100°C in a boiling water bath results in a total loss of activity | Zea mays |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Zea mays |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome P-450 | dependent on | Zea mays |
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Release 2023.1 (January 2023)
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