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Literature summary for 1.14.14.108 extracted from

  • Yu, C.A.; Gunsalus, I.C.
    Monoxygenases. VII. Camphor ketolactonase I and the role of three protein components (1969), J. Biol. Chem., 244, 6149-6152.
    View publication on PubMed
Search for more literature for 1.14.14.108

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
 FMN component E1 Pseudomonas putida
0.001
-
 FAD component E1 Pseudomonas putida

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Pseudomonas putida 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 component E1, analytical centrifugation Pseudomonas putida
80000
-
 component E2, analytical centrifugation Pseudomonas putida
120000
-
 component E3, analytical centrifugation Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-bornane-2,5-dione + FMNH2 + O2 Pseudomonas putida i.e. 2,5-diketocamphane, reaction in camphor catabolism of Pseudomonas putida ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
 strain PpG786, mutant derived from strain PpG1
-

Purification (Commentary)

Purification (Comment) Organism
separation of components E1, E2, E3 Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10 20 component E3, ketolactonizing Pseudomonas putida
24
-
 component E2 Pseudomonas putida
420
-
 purified component E1, FMN-coupled NADH-dehydrogenase Pseudomonas putida

Storage Stability

Storage Stability Organism
-20°C, ammonium sulfate precipitates as frozen pastes stable over a long period of time Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-5-oxo-1,2-campholide + FAD + H2O
-
 Pseudomonas putida (+)-bornane-2,5-dione + FADH2 + O2
-
 r
(+)-bornane-2,5-dione + FMNH2 + O2 i.e. 2,5-diketocamphane Pseudomonas putida 3,4,4-trimethyl-5-carboxy-methyl-DELTA2-cyclopentenone + FMN + H2O i.e. cyclopentenoic acid ?
(+)-bornane-2,5-dione + FMNH2 + O2 i.e. 2,5-diketocamphane, reaction in camphor catabolism of Pseudomonas putida Pseudomonas putida ?
-
 ?
additional information rubredoxin not mentioned Pseudomonas putida ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Pseudomonas putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 10 min, component E2 retains full activity, component E3 loses 50%, component E3 loses 90% in the presence of substrate or component E1 Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
20
-
 NADH component E3 Pseudomonas putida
31.7
-
 NADH component E2 Pseudomonas putida
53.3
-
 NADH component E1 Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
 Pseudomonas putida

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 80% of activity maximum at pH 6.5 and pH 8.5 Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
FAD 2 mol per mol of component E3 Pseudomonas putida
FMN 0.4 mol per mol of component E1, 0.7 mol per mol of component E2 Pseudomonas putida
FMN components E1 and E2 bind FMN reversibly Pseudomonas putida