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Literature summary for 1.14.14.107 extracted from

  • Wang, C.; Bai, M.; Tian, Y.; Tian, W.
    Characterisation of two genes encoding cytochrome P450 monooxygenases involved in gibberellin biosynthesis in apple (Malus x domestica Borkh.) (2014), J. Hortic. Sci. Biotechnol., 89, 329-337 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
gene MdKAO, DNA and amino acid sequence determination and analysis, the sequence contains 7 introns, location on chromosome 2, quantitative real-time PCR enzyme expression analysis Malus domestica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ent-kaur-16-en-19-oate + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 Malus domestica overall reaction gibberellin A12 + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
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Organism

Organism UniProt Comment Textmining
Malus domestica M9R757 cv. Fuji
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Source Tissue

Source Tissue Comment Organism Textmining
additional information the enzyme level of MdKAO is significantly lower in the columnar parent, and in F1 progeny with the columnar phenotype, compared to standard parent and F1 progeny with the standard phenotype Malus domestica
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ent-kaur-16-en-19-oate + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 overall reaction Malus domestica gibberellin A12 + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
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?

Synonyms

Synonyms Comment Organism
KAO
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Malus domestica

Cofactor

Cofactor Comment Organism Structure
cytochrome P-450
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Malus domestica
NADPH-hemoprotein reductase A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ Malus domestica

General Information

General Information Comment Organism
physiological function lower expression of the MdKAO gene is responsible for the reduced concentrations of bioactive gibberellins in the apical tissue of columnar apple stems Malus domestica