Wang, C.; Bai, M.; Tian, Y.; Tian, W.
Characterisation of two genes encoding cytochrome P450 monooxygenases involved in gibberellin biosynthesis in apple (Malus x domestica Borkh.) (2014), J. Hortic. Sci. Biotechnol., 89, 329-337 .
No PubMed abstract available
Cloned(Commentary)
Cloned (Comment) |
Organism |
gene MdKAO, DNA and amino acid sequence determination and analysis, the sequence contains 7 introns, location on chromosome 2, quantitative real-time PCR enzyme expression analysis |
Malus domestica |
Natural Substrates/ Products (Substrates)
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
ent-kaur-16-en-19-oate + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 |
Malus domestica |
overall reaction |
gibberellin A12 + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O |
- |
? |
|
Organism
Organism |
UniProt |
Comment |
Textmining |
Malus domestica |
M9R757 |
cv. Fuji |
- |
Source Tissue
Source Tissue |
Comment |
Organism |
Textmining |
additional information |
the enzyme level of MdKAO is significantly lower in the columnar parent, and in F1 progeny with the columnar phenotype, compared to standard parent and F1 progeny with the standard phenotype |
Malus domestica |
- |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
ent-kaur-16-en-19-oate + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 |
overall reaction |
Malus domestica |
gibberellin A12 + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O |
- |
? |
|
Synonyms
Synonyms |
Comment |
Organism |
KAO |
- |
Malus domestica |
Cofactor
Cofactor |
Comment |
Organism |
Structure |
cytochrome P-450 |
- |
Malus domestica |
|
NADPH-hemoprotein reductase |
A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ |
Malus domestica |
|
General Information
General Information |
Comment |
Organism |
physiological function |
lower expression of the MdKAO gene is responsible for the reduced concentrations of bioactive gibberellins in the apical tissue of columnar apple stems |
Malus domestica |