Literature summary for 1.14.14.106 extracted from

DeJong, J.H.; Liu, Y.; Bollon, A.P.; Long, R.M.; Jennewein, S.; Williams, D.; Croteau, R.B.
Genetic engineering of taxol biosynthetic genes in Saccharomyces cerevisiae (2006), Biotechnol. Bioeng., 93, 212-224.

Search for more literature for 1.14.14.106

Cloned(Commentary)

Cloned (Comment)	Organism
expression of the VSVG-tagged enzyme in Saccharomyces cerevisiae YPH499 cells, expression of the enzymes from and reconstruction of the taxol biosynthesis pathway 5 early steps by expression of the participating enzymes in yeast, overview	Taxus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
taxa-4(20),11(12)-dien-5alpha-yl acetate + [reduced NADPH-hemoprotein reductase] + O2	Taxus sp.	step in taxol biosynthesis, metabolite analysis and pathway overview	taxa-4(20),11(12)-dien-5alpha-yl-acetyl-13alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Taxus sp.	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant VSVG-tagged enzyme from Saccharomyces cerevisiae YPH499 cells	Taxus sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
taxa-4(20),11(12)-dien-5alpha-yl acetate + [reduced NADPH-hemoprotein reductase] + O2	-	Taxus sp.	taxa-4(20),11(12)-dien-5alpha-yl-acetyl-13alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O	product identification and quantification by HPLC and GC-MS	?
taxa-4(20),11(12)-dien-5alpha-yl acetate + [reduced NADPH-hemoprotein reductase] + O2	step in taxol biosynthesis, metabolite analysis and pathway overview	Taxus sp.	taxa-4(20),11(12)-dien-5alpha-yl-acetyl-13alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O	-	?

Synonyms

Synonyms	Comment	Organism
taxoid 13alpha-hydroxylase	-	Taxus sp.
THY13a	-	Taxus sp.

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
31	-	assay at	Taxus sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Taxus sp.

Cofactor

Cofactor	Comment	Organism	Structure
cytochrome P-450	-	Taxus sp.

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Release 2023.1 (January 2023)