Cloned (Comment) | Organism |
---|---|
isolation and cloning of a single-copy gene, DNA and amino acid sequence determination and analysis, expression in insect cells, functional co-expression of full-length and truncated enzyme with taxoid 13beta-hydroxylase or with the organisms' taxoid 10beta-hydroxylase in Saccharomyces cerevisiae strain YPH499, subcloning in Escherichia coli | Taxus cuspidata |
isolation and cloning of a single-copy gene, DNA and amino acid sequence determination and analysis, functional co-expression with the NADPH:cytochrome P450 reductase, also exhibiting taxoid 10beta-hydroxylase activity, in Saccharomyces cerevisiae strain YPH499, subcloning in Escherichia coli | Taxus cuspidata |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a truncated enzyme version | Taxus cuspidata |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Taxus cuspidata | 5783 | - |
microsome | - |
Taxus cuspidata | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | heme iron | Taxus cuspidata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
taxadien-5alpha-yl acetate + [reduced NADPH-hemoprotein reductase] + O2 | Taxus cuspidata | the enzyme is involved in the taxol biosynthetic pathway, overview | 10beta-hydroxytaxa-4(20),11-dien-5alpha-yl acetate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Taxus cuspidata | Q9AXM6 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Taxus cuspidata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cytochrome c + FAD + FMN + O2 | rat cytochrome b5 | Taxus cuspidata | ? | - |
? | |
taxadien-5alpha-yl acetate + [reduced NADPH-hemoprotein reductase] + O2 | - |
Taxus cuspidata | 10beta-hydroxytaxa-4(20),11-dien-5alpha-yl acetate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
taxadien-5alpha-yl acetate + [reduced NADPH-hemoprotein reductase] + O2 | the enzyme is involved in the taxol biosynthetic pathway, overview | Taxus cuspidata | 10beta-hydroxytaxa-4(20),11-dien-5alpha-yl acetate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cytochrome P450 reductase | - |
Taxus cuspidata |
NADPH:cytochrome P450 reductase | - |
Taxus cuspidata |
taxoid 10beta-hydroxylase | - |
Taxus cuspidata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
assay at | Taxus cuspidata |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P-450 | - |
Taxus cuspidata | |
FAD | - |
Taxus cuspidata | |
FMN | - |
Taxus cuspidata |