Cloned (Comment) | Organism |
---|---|
gene LOC104585940 encoding isozyme 2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative RT-PCR enzyme expression analysis | Nelumbo nucifera |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2 | Nelumbo nucifera | - |
(S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nelumbo nucifera | A0A1U7Z269 | isozyme 2; 2 isozymes, 2 cultivars | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Nelumbo nucifera | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2 | - |
Nelumbo nucifera | (S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
(S)-N-methylcoclaurine-3-hydroxylase | - |
Nelumbo nucifera |
CYP80B | - |
Nelumbo nucifera |
LOC104585940 | locus name | Nelumbo nucifera |
N-methylcoclaurine 3'-hydroxylase | - |
Nelumbo nucifera |
NMCH | - |
Nelumbo nucifera |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P-450 | - |
Nelumbo nucifera | |
NADPH-hemoprotein reductase | A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ | Nelumbo nucifera |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the common pathway of benzylisoquinoline alkaloid biosynthesis and the aporphine alkaloid bisynthesis pathway in lotus, overview. A series of enzymes, norcoclaurine 6-O-methyltransferase (6OMT), coclaurineN-methyltransferase (CNMT), (S)-N-methylcoclaurine-3-hydroxylase (NMCH), and 3-hydroxy-N-methylcoclaurine 4'-O-methyltransferase(4'OMT), exhibit catalytic activity to yield the central branchpoint intermediate (S)-reticuline | Nelumbo nucifera |
physiological function | the expression of isozyme 1 is low in the two cultivars, so it may have little effect on alkaloid contents. The expression of CYP80B, NNU08355, has a positive effect on aporphine alkaloids content in lotus, but exhibits phenotypic lag, which might be because of NMCH which acts early in the benzylisoquinoline alkaloid biosynthetic pathway | Nelumbo nucifera |