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Literature summary for 1.14.14.10 extracted from

  • Payne, J.W.; Bolton, H.; Campbell, J.A.; Xun, L.
    Purification and characterization of EDTA monooxygenase from the EDTA-degrading bacterium BNC1 (1998), J. Bacteriol., 180, 3823-3827.
    View publication on PubMedView publication on EuropePMC

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ EDTA monooxygenase degrades nitrilotriacetate in the absence or presence of Mg2+ Chelativorans sp. BNC1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
gel filtration Chelativorans sp. BNC1

Organism

Organism UniProt Comment Textmining
Chelativorans sp. BNC1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Blue 3GA agarose column chromatography, MonoQ HR 5/5 column chromatography, and hydroxyapatite column chromatography Chelativorans sp. BNC1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
EDTA + O2 + FMNH2 + H+
-
Chelativorans sp. BNC1 ethylenediaminetriacetate + glyoxylate + H2O + FMN
-
?
nitrilotriacetate + O2 + FMNH2 + H+
-
Chelativorans sp. BNC1 iminodiacetate + glyoxylate + H2O + FMN
-
?

Subunits

Subunits Comment Organism
monomer 1 * 45000, SDS-PAGE Chelativorans sp. BNC1

Synonyms

Synonyms Comment Organism
EDTA monooxygenase
-
Chelativorans sp. BNC1
NTA-MO
-
Chelativorans sp. BNC1

Cofactor

Cofactor Comment Organism Structure
FMNH2
-
Chelativorans sp. BNC1