Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ni2+ | ionic or uncomplexed Ni2+ (2 mM) is inhibitory to the enzyme | Aminobacter aminovorans | |
Zn2+ | ionic or uncomplexed Zn2+ (2 mM) is inhibitory to the enzyme | Aminobacter aminovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0317 | - |
nitrilotriacetate | with equimolar Ni2+ (0.04-0.2 mM), in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C | Aminobacter aminovorans | |
0.0367 | - |
nitrilotriacetate | with equimolar Co2+ (0.04-0.2 mM), in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C | Aminobacter aminovorans | |
0.0404 | - |
nitrilotriacetate | with equimolar Mg2+ (0.04-0.2 mM), in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C | Aminobacter aminovorans | |
0.043 | - |
nitrilotriacetate | with 2 mM Mg2+and varying nitrilotriacetate concentrations, in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C | Aminobacter aminovorans | |
0.0576 | - |
nitrilotriacetate | with equimolar Zn2+ (0.04-0.2 mM), in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C | Aminobacter aminovorans | |
0.0905 | - |
nitrilotriacetate | with equimolar Mn2+ (0.04-0.2 mM), in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C | Aminobacter aminovorans | |
0.0968 | - |
nitrilotriacetate | with equimolar Fe2+ (0.04-0.2 mM), in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C | Aminobacter aminovorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Al3+ | nitrilotriacetate is only a substrate when complexed with cations such as Mg2+, Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+ | Aminobacter aminovorans | |
Co2+ | nitrilotriacetate is only a substrate when complexed with cations such as Mg2+, Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+ | Aminobacter aminovorans | |
Cu2+ | nitrilotriacetate is only a substrate when complexed with cations such as Mg2+, Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+ | Aminobacter aminovorans | |
Fe2+ | nitrilotriacetate is only a substrate when complexed with cations such as Mg2+, Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+ | Aminobacter aminovorans | |
Mg2+ | the enzyme shows little preference for Mg-nitrilotriacetate over Co-nitrilotriacetate, Ni-nitrilotriacetate, and Zn-nitrilotriacetate | Aminobacter aminovorans | |
Ni2+ | nitrilotriacetate is only a substrate when complexed with cations such as Mg2+, Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+ | Aminobacter aminovorans | |
Zn2+ | nitrilotriacetate is only a substrate when complexed with cations such as Mg2+, Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+ | Aminobacter aminovorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aminobacter aminovorans | - |
- |
- |
Aminobacter aminovorans ATCC 29600 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partially purified by ammonium sulfate precipitation and phenyl agarose column chromatography | Aminobacter aminovorans |
Storage Stability | Organism |
---|---|
-80°C, partially purified, dialyzed enzyme, 3 months, no loss of activity | Aminobacter aminovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | nitrilotriacetate not complexed with cation is not degraded. The addition of Cr2+,Ba2+, Sr2+, Cr3+, K+, or Na+ results in no degradation of nitrilotriacetate | Aminobacter aminovorans | ? | - |
? | |
additional information | nitrilotriacetate not complexed with cation is not degraded. The addition of Cr2+,Ba2+, Sr2+, Cr3+, K+, or Na+ results in no degradation of nitrilotriacetate | Aminobacter aminovorans ATCC 29600 | ? | - |
? | |
nitrilotriacetate + FMNH2 + H+ + O2 | nitrilotriacetate is only a substrate when complexed with cations such as Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+. The enzyme shows little preference for Mg-nitrilotriacetate over Co-nitrilotriacetate, Ni-nitrilotriacetate, and Zn-nitrilotriacetate | Aminobacter aminovorans | iminodiacetate + glyoxylate + FMN + H2O | - |
? | |
nitrilotriacetate + FMNH2 + H+ + O2 | nitrilotriacetate is only a substrate when complexed with cations such as Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+. The enzyme shows little preference for Mg-nitrilotriacetate over Co-nitrilotriacetate, Ni-nitrilotriacetate, and Zn-nitrilotriacetate | Aminobacter aminovorans ATCC 29600 | iminodiacetate + glyoxylate + FMN + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NTA-MO | - |
Aminobacter aminovorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMNH2 | - |
Aminobacter aminovorans |