Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.14.1 extracted from

  • Zhang, L.; Xie, Z.; Liu, Z.; Zhou, S.; Ma, L.; Liu, W.; Huang, J.; Ko, T.; Li, X.; Hu, Y.; Min, J.; Yu, X.; Guo, R.; Chen, C.
    Structural insight into the electron transfer pathway of a self-sufficient P450 monooxygenase (2020), Nat. Commun., 11, 2676 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
structure of full-length CYP116B46. The continuous polypeptide chain comprises three functional domains, which align with the direction of electrons traveling from FMN to the heme through the [2Fe-2S] cluster. FMN and the [2Fe-2S] cluster are positioned closely, which facilitates efficient electron shuttling. The edge-to-edge straight-line distance between the [2Fe-2S] cluster and heme is approx. 25.3 A Tepidiphilus thermophilus

Protein Variants

Protein Variants Comment Organism
E723A residue located between [2Fe-2S] cluster and heme, about 15% of wild-type activity Tepidiphilus thermophilus
E729A residue located between [2Fe-2S] cluster and heme, no activity detecable Tepidiphilus thermophilus
F378A residue located between [2Fe-2S] cluster and heme, no activity detecable Tepidiphilus thermophilus
Q725A residue located between [2Fe-2S] cluster and heme, about 100% of wild-type activity Tepidiphilus thermophilus
R388A residue located between [2Fe-2S] cluster and heme, no activity detecable Tepidiphilus thermophilus
R392A residue located between [2Fe-2S] cluster and heme, about 110% of wild-type activity Tepidiphilus thermophilus
R718A residue located between [2Fe-2S] cluster and heme, no activity detecable Tepidiphilus thermophilus
S726A residue located between [2Fe-2S] cluster and heme, about 30% of wild-type activity Tepidiphilus thermophilus

Organism

Organism UniProt Comment Textmining
Tepidiphilus thermophilus A0A0K6ITW2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
decanoic acid + [reduced NADPH-hemoprotein reductase] + O2
-
Tepidiphilus thermophilus ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Synonyms

Synonyms Comment Organism
CYP116B46
-
Tepidiphilus thermophilus

Cofactor

Cofactor Comment Organism Structure
heme
-
Tepidiphilus thermophilus
[2Fe-2S]-center
-
Tepidiphilus thermophilus