Crystallization (Comment) | Organism |
---|---|
structure of full-length CYP116B46. The continuous polypeptide chain comprises three functional domains, which align with the direction of electrons traveling from FMN to the heme through the [2Fe-2S] cluster. FMN and the [2Fe-2S] cluster are positioned closely, which facilitates efficient electron shuttling. The edge-to-edge straight-line distance between the [2Fe-2S] cluster and heme is approx. 25.3 A | Tepidiphilus thermophilus |
Protein Variants | Comment | Organism |
---|---|---|
E723A | residue located between [2Fe-2S] cluster and heme, about 15% of wild-type activity | Tepidiphilus thermophilus |
E729A | residue located between [2Fe-2S] cluster and heme, no activity detecable | Tepidiphilus thermophilus |
F378A | residue located between [2Fe-2S] cluster and heme, no activity detecable | Tepidiphilus thermophilus |
Q725A | residue located between [2Fe-2S] cluster and heme, about 100% of wild-type activity | Tepidiphilus thermophilus |
R388A | residue located between [2Fe-2S] cluster and heme, no activity detecable | Tepidiphilus thermophilus |
R392A | residue located between [2Fe-2S] cluster and heme, about 110% of wild-type activity | Tepidiphilus thermophilus |
R718A | residue located between [2Fe-2S] cluster and heme, no activity detecable | Tepidiphilus thermophilus |
S726A | residue located between [2Fe-2S] cluster and heme, about 30% of wild-type activity | Tepidiphilus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tepidiphilus thermophilus | A0A0K6ITW2 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
decanoic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Tepidiphilus thermophilus | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP116B46 | - |
Tepidiphilus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Tepidiphilus thermophilus | |
[2Fe-2S]-center | - |
Tepidiphilus thermophilus |