Application | Comment | Organism |
---|---|---|
synthesis | coexpression of P450 reductase with Sam5 in Escherichia coli enhances hydroxylation by approximately from 34 to 50%, depending on the flavonoid used. Optimization of the production of bioactive flavonoids, 8-hydroxyluteolin and 3'-hydroxydaidzein, in this system gives 88 mg/l of 8-hydroxyluteolin and 75 mg/l of 3'-hydroxydaidzein | Saccharothrix espanaensis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharothrix espanaensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharothrix espanaensis | Q2EYY8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
apigenin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Saccharothrix espanaensis | 3',8-dihydroxyapigenin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
daidzein + [reduced NADPH-hemoprotein reductase] + O2 | - |
Saccharothrix espanaensis | 3'-hydroxydaidzein + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
eriodictyol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Saccharothrix espanaensis | 6-hydroxyeriodictyol + 8-hydroxyeriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
genistein + [reduced NADPH-hemoprotein reductase] + O2 | - |
Saccharothrix espanaensis | 3',8-dihydroxygenistein + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
luteolin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Saccharothrix espanaensis | 3',8-dihydroxyapigenin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
additional information | isoform Sam5 hydroxylates isoflavones, flavanones, and flavones but does not produce any detectable hydroxylated product with flavonols. Naringenin is the most preferred substrate, followed by eriodictyol, genistein,apigenin, luteolin, and daidzein | Saccharothrix espanaensis | ? | - |
? | |
naringenin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Saccharothrix espanaensis | eriodictyol + 6-hydroxyeriodictyol + 8-hydroxyeriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Sam5 | - |
Saccharothrix espanaensis |