Literature summary for 1.14.14.1 extracted from

Wriessnegger, T.; Moser, S.; Emmerstorfer-Augustin, A.; Leitner, E.; Mueller, M.; Kaluzna, I.; Schuermann, M.; Mink, D.; Pichler, H.
Enhancing cytochrome P450-mediated conversions in P. pastoris through RAD52 over-expression and optimizing the cultivation conditions (2016), Fungal Genet. Biol., 89, 114-125 .

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Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	the enzyme shows significantly enhanced activity upon over-expression and cultivation optimization of the DNA repair and recombination protein RAD52. RAD52 overexpression in Pichia pastoris CYP2D6-hCPR leads to twofold enhanced 1'-hydroxy bufuralol formation after cultivation and induction at pH 6, while shifting cultivation and induction to pH 8 is similarly effective. The beneficial effects are largely additive yielding in about 3fold more bufuralol hydroxylation in Pichia pastoris CYP2D6-hCPR-RAD52 than in the initial strain and condition	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in Pichia pastoris. RAD52 overexpression in Pichia pastoris CYP2D6-hCPR leads to twofold enhanced 1'-hydroxy bufuralol formation after cultivation and induction at pH 6, while shifting cultivation and induction to pH 8 is similarly effective. The beneficial effects are largely additive yielding in about 3fold more bufuralol hydroxylation in Pichia pastoris CYP2D6-hCPR-RAD52 than in the initial strain and condition	Homo sapiens

Cloned (Comment)

Organism

overexpression in Pichia pastoris. RAD52 overexpression in Pichia pastoris CYP2D6-hCPR leads to twofold enhanced 1'-hydroxy bufuralol formation after cultivation and induction at pH 6, while shifting cultivation and induction to pH 8 is similarly effective. The beneficial effects are largely additive yielding in about 3fold more bufuralol hydroxylation in Pichia pastoris CYP2D6-hCPR-RAD52 than in the initial strain and condition

Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P10635	-	-

Synonyms

Synonyms	Comment	Organism
CP2D6	-	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
cytochrome P450	a heme-thiolate protein (P-450)	Homo sapiens

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Release 2023.1 (January 2023)