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Literature summary for 1.14.14.1 extracted from

  • Syed, K.; Porollo, A.; Miller, D.; Yadav, J.
    Rational engineering of the fungal P450 monooxygenase CYP5136A3 to improve its oxidizing activity toward polycyclic aromatic hydrocarbons (2013), Protein Eng., 26, 553-557.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain KM71H Phanerodontia chrysosporium

Protein Variants

Protein Variants Comment Organism
W129A/L324G the mutant shows complete loss of activity toward pyrene and retains partial oxidation activity (32%) toward phenanthrene Phanerodontia chrysosporium
W129F/L324F the mutant shows complete loss of activity toward both phenanthrene and pyrene Phanerodontia chrysosporium
W129F/L324G the mutant shows complete loss of activity toward pyrene and retains partial oxidation activity (15%) toward phenanthrene Phanerodontia chrysosporium
W129L/L324F the mutant shows greater oxidation of phenanthrene and pyrene compared with the wild type enzyme Phanerodontia chrysosporium
W129L/L324G the mutant shows complete loss of activity toward both phenanthrene and pyrene Phanerodontia chrysosporium

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phenanthrene + [reduced NADPH-hemoprotein reductase] + O2
-
 Phanerodontia chrysosporium ?
-
 ?
pyrene + [reduced NADPH-hemoprotein reductase] + O2
-
 Phanerodontia chrysosporium ?
-
 ?

Synonyms

Synonyms Comment Organism
CYP5136A3
-
 Phanerodontia chrysosporium
cytochrome P450 monooxygenase
-
 Phanerodontia chrysosporium