Cloned (Comment) | Organism |
---|---|
CYP82E2, DNA and amino acid sequence determination and analysis, microarray-based strategy to identify genes that are differentially regulated between closely related tobacco lines that accumulate either nicotine, termed nonconverters, or nornicotine, termed converters, as the predominant alkaloid in the cured leaf, expression in Saccharomyces cerevisiae strain WAT11, overexpression in transgenic tobacco plants | Nicotiana tabacum |
CYP82E3, DNA and amino acid sequence determination and analysis, microarray-based strategy to identify genes that are differentially regulated between closely related tobacco lines that accumulate either nicotine, termed nonconverters, or nornicotine, termed converters, as the predominant alkaloid in the cured leaf, expression in Saccharomyces cerevisiae strain WAT11, overexpression in transgenic tobacco plants | Nicotiana tabacum |
CYP82E4v1, DNA and amino acid sequence determination and analysis, microarray-based strategy to identify genes that are differentially regulated between closely related tobacco lines that accumulate either nicotine, termed nonconverters, or nornicotine, termed converters, as the predominant alkaloid in the cured leaf, expression in Saccharomyces cerevisiae strain WAT11, overexpression in transgenic tobacco plants | Nicotiana tabacum |
CYP82E4v2, DNA and amino acid sequence determination and analysis, microarray-based strategy to identify genes that are differentially regulated between closely related tobacco lines that accumulate either nicotine, termed nonconverters, or nornicotine, termed converters, as the predominant alkaloid in the cured leaf, expression in Saccharomyces cerevisiae strain WAT11 | Nicotiana tabacum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of transgenic plants overexpressing the enzyme, analysis of alkaloid spectrum, overview | Nicotiana tabacum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Nicotiana tabacum | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Nicotiana tabacum | the enzyme demethylates nicotine to nornicotine, nornicotine serves as the precursor in the synthesis of the well characterized carcinogen N'-nitrosonornicotine during the curing and processing of tobacco | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | Q38Q84 | CYP82E3 | - |
Nicotiana tabacum | Q38Q85 | CYP82E2 | - |
Nicotiana tabacum | Q38Q86 | CYP82E4v1 | - |
Nicotiana tabacum | Q38Q87 | CYP82E4v2; three CYP82E isozymes | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Nicotiana tabacum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme demethylates nicotine to nornicotine, nornicotine serves as the precursor in the synthesis of the well characterized carcinogen N'-nitrosonornicotine during the curing and processing of tobacco | Nicotiana tabacum | ? | - |
? | |
additional information | the enzyme demethylates nicotine to nornicotine | Nicotiana tabacum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP82E2 | - |
Nicotiana tabacum |
CYP82E3 | - |
Nicotiana tabacum |
CYP82E4v1 | - |
Nicotiana tabacum |
CYP82E4v2 | - |
Nicotiana tabacum |
cytochrome P450 monooxygenase | - |
Nicotiana tabacum |
More | the enzyme belongs to the CYP82E2 family | Nicotiana tabacum |