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Literature summary for 1.14.14.1 extracted from
- Substrate specificity of native and mutated cytochrome P450 (CYP102A3) from Bacillus subtilis (2004), J. Biotechnol., 108, 41-49.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dimethylsulfoxid | up to 25% v/v | Bacillus subtilis |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A74G/F88V | substrate specificity similar to wild-type | Bacillus subtilis |
| A74G/F88V/S189Q | substrate spectrum much broader than for wild-type | Bacillus subtilis |
| A74G/S189Q | substrate specificity similar to wild-type | Bacillus subtilis |
General Stability
| General Stability | Organism |
|---|---|
| not stable in NaCl above 150 mM | Bacillus subtilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0064 | - |
omega-(p-nitrophenyl)decanoic acid | pH 8.0, mutant A74G/F88V/S189Q | Bacillus subtilis | |
| 0.0065 | - |
omega-(p-nitrophenyl)dodecanoic acid | pH 8.0, mutant A74G/F88V/S189Q | Bacillus subtilis | |
| 0.0104 | - |
omega-(p-nitrophenyl)dodecanoic acid | pH 8.0, wild-type | Bacillus subtilis | |
| 0.0245 | - |
omega-(p-nitrophenyl)decanoic acid | pH 8.0, wild-type | Bacillus subtilis | |
| 0.0319 | - |
omega-(p-nitrophenyl)octanoic acid | pH 8.0, mutant A74G/F88V/S189Q | Bacillus subtilis | |
| 0.0618 | - |
omega-(p-nitrophenyl)octanoic acid | pH 8.0, wild-type | Bacillus subtilis | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| DMSO | stable, and activity increases in presence of up to 25% DMSO (v/v) | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
isozyme CYP102A3, natural fusion protein consisting of a heme domain and a reductase domain | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-ionone + [reduced NADPH-hemoprotein reductase] + O2 | substrate only for mutant A74G/F88V/S189Q | Bacillus subtilis | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| capric acid + [reduced NADPH-hemoprotein reductase] + O2 | about 10% of the activity with palmitic acid, wild-type | Bacillus subtilis | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| caprylic acid + [reduced NADPH-hemoprotein reductase] + O2 | substrate only for mutant A74G/F88V/S189Q | Bacillus subtilis | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| lauric acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Bacillus subtilis | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| naphthalene + [reduced NADPH-hemoprotein reductase] + O2 | substrate only for mutant A74G/F88V/S189Q | Bacillus subtilis | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| octane + [reduced NADPH-hemoprotein reductase] + O2 | substrate only for mutant A74G/F88V/S189Q and for mutant A74G/S189Q | Bacillus subtilis | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| omega-(p-nitrophenyl)decanoic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Bacillus subtilis | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| omega-(p-nitrophenyl)dodecanoic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Bacillus subtilis | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| omega-(p-nitrophenyl)octanoic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Bacillus subtilis | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| palmitic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Bacillus subtilis | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
omega-(p-nitrophenyl)octanoic acid | pH 8.0, wild-type | Bacillus subtilis | |
| 1.3 | - |
omega-(p-nitrophenyl)dodecanoic acid | pH 8.0, mutant A74G/F88V/S189Q | Bacillus subtilis | |
| 1.3 | - |
omega-(p-nitrophenyl)decanoic acid | pH 8.0, mutant A74G/F88V/S189Q | Bacillus subtilis | |
| 1.5 | - |
omega-(p-nitrophenyl)decanoic acid | pH 8.0, wild-type | Bacillus subtilis | |
| 3.1 | - |
omega-(p-nitrophenyl)dodecanoic acid | pH 8.0, wild-type | Bacillus subtilis | |
| 3.5 | - |
omega-(p-nitrophenyl)octanoic acid | pH 8.0, mutant A74G/F88V/S189Q | Bacillus subtilis | |
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