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Literature summary for 1.14.14.1 extracted from

  • Eschenfeldt, W.H.; Zhang, Y.; Samaha, H.; Stols, L.; Eirich, L.D.; Wilson, C.R.; Donnelly, M.I.
    Transformation of fatty acids catalyzed by cytochrome P450 monooxygenase enzymes of Candida tropicalis (2003), Appl. Environ. Microbiol., 69, 5992-5999.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.1

Cloned(Commentary)

Cloned (Comment) Organism
both isozymes CYP52A13, CYP52A17 Candida tropicalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.023
-
 myristic acid isozyme CYP52A17, pH 7.4, 35°C Candida tropicalis
0.075
-
 oleic acid isozyme CYP52A17, pH 7.4, 35°C Candida tropicalis
0.084
-
 oleic acid isozyme CYP52A13, pH 7.4, 35°C Candida tropicalis
0.11
-
 myristic acid isozyme CYP52A13, pH 7.4, 35°C Candida tropicalis

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Candida tropicalis
-
-

Organism

Organism UniProt Comment Textmining
Candida tropicalis
-
 ATCC 20336, isozymes CYP52A13, CYP52A17
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
decanoic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Candida tropicalis ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
dodecanoic acid + [reduced NADPH-hemoprotein reductase] + O2 isozyme CYP52A17, good substrate Candida tropicalis ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
eicosenoic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Candida tropicalis ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
linoleic acid + [reduced NADPH-hemoprotein reductase] + O2 isozyme CYP52A17, good substrate, isozyme CYP52A13, good substrate Candida tropicalis ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
myristic acid + [reduced NADPH-hemoprotein reductase] + O2 isozyme CYP52A17, best substrate Candida tropicalis omega-hydroxy-myristic acid + [oxidized NADPH-hemoprotein reductase] + H2O isoform CYP52A17, additional fornmation of about 15% dicarbonic acid ?
oleic acid + [reduced NADPH-hemoprotein reductase] + O2 isozyme CYP52A13, best substrate,isozyme CYP52A17, good substrate Candida tropicalis omega-hydroxy oleic acid + n-decan-dicarboxylic acid + [oxidized NADPH-hemoprotein reductase] + H2O isozyme CYP52A13, products in ratio 1:1, CYP52A17, ratio 3:7 of hydroxy- and dicarboxy-product ?
palmitic acid + [reduced NADPH-hemoprotein reductase] + O2 isozyme CYP52A17, good substrate Candida tropicalis ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
stearic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Candida tropicalis ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
-
 Candida tropicalis
NADPH
-
 Candida tropicalis