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Literature summary for 1.14.13.92 extracted from
- Laboratory evolution of robust and enantioselective Baeyer-Villiger monooxygenases for asymmetric catalysis (2009), J. Am. Chem. Soc., 131, 15424-15432.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Top10 cells | Thermobifida fusca |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| P440F | higher subtrate variability, temperature optimum at 50C with range from 45-56C | Thermobifida fusca |
| P440H | higher subtrate variability, temperature optimum at 50C with range from 45-56C | Thermobifida fusca |
| P440I | higher subtrate variability, temperature optimum at 50C with range from 45-56C | Thermobifida fusca |
| P440L | higher subtrate variability, temperature optimum at 50C with range from 45-56C | Thermobifida fusca |
| P440N | higher subtrate variability, temperature optimum at 50C with range from 45-56C | Thermobifida fusca |
| P440T | higher subtrate variability, temperature optimum at 50C with range from 45-56C | Thermobifida fusca |
| P440Y | higher subtrate variability, temperature optimum at 50C with range from 45-58C | Thermobifida fusca |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermobifida fusca | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermobifida fusca |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phenylacetone + NADPH + O2 | reaction is performed in presence of 2 U secondary alcohol dehydrogenase from Thermoanaerobacter ethanolicus and isopropanol to recover NADPH | Thermobifida fusca | benzyl acetate + NADP+ + H2O | - |
? |  |
| rac-2-ethylcyclohexanone + NADPH + O2 | substrate is only accepted by mutants of phenylacetone monooxygenase, reaction is performed in presence of 2 U secondary alcohol dehydrogenase from Thermoanaerobacter ethanolicus and isopropanol to recover NADPH | Thermobifida fusca | benzyl acetate + NADP+ + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Baeyer-Villiger monooxygenase | - |
Thermobifida fusca |
| BVMO | - |
Thermobifida fusca |
| PAMO | - |
Thermobifida fusca |
| phenylacetone monooxygenase | - |
Thermobifida fusca |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 56 | - |
- |
Thermobifida fusca |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 58 | at 58C 75% of enzyme activity remains, at 60C activity is nearly zero, at 45C enzyme is fully active | Thermobifida fusca |
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