Activating Compound | Comment | Organism | Structure |
---|---|---|---|
hexane | enzyme activity is highest at concentrations of hexane between 5% and 30% | Pseudomonas fluorescens |
Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli | Thermobifida fusca |
overexpressed via plasmid in Escherichia coli | Pseudomonas fluorescens |
Protein Variants | Comment | Organism |
---|---|---|
M446G | enzyme has altered activity and substrate specificity | Thermobifida fusca |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hexane | enzyme activity is markedly reduced at concentrations of hexane below 5% and over 30% | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Thermobifida fusca | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas fluorescens |
- |
Thermobifida fusca |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-bromo-indanone + NADPH + O2 | substrate was only accepted by phenylacetone monooxygenase of Pseudomonas fluorescens but not of Thermobifida fusca, reaction was performed in presence of 10 U glucose-6-phosphate dehydrogenase and glucose-6-phosphate to recover NADPH | Pseudomonas fluorescens | 6-bromoisochroman-1-one + NADP+ + H2O | - |
? | |
1-indanone + NADPH + H+ + O2 | substrate was only accepted by phenylacetone monooxygenase of Pseudomonas fluorescens but not of Thermobifida fusca, reaction was performed in presence of 10 U glucose-6-phosphate dehydrogenase and glucose-6-phosphate to recover NADPH | Pseudomonas fluorescens | 3,4-dihydrocoumarin + NADP+ + H2O | - |
? | |
1-indanone + NADPH + H+ + O2 | reaction product is only synthesized by the mutant M446G of phenylacetone monooxygenase of Thermobifida fusca, reaction is performed in presence of 10 U glucose-6-phosphate dehydrogenase and glucose-6-phosphate to recover NADPH | Thermobifida fusca | 1-isochromanone + NADP+ + H2O | - |
? | |
1-tetralone + NADPH + O2 | substrate was accepted by phenylacetone monooxygenase of Pseudomonas fluorescens but not of Thermobifida fusca, reaction was performed in presence of 10 U glucose-6-phosphate dehydrogenase and glucose-6-phosphate to recover NADPH | Pseudomonas fluorescens | 4,5-dihydro-1-benzoxepin-2(3H)-one + NADP+ + H2O | - |
? | |
2-indanone + NADPH + H+ + O2 | substrate is only accepted by the mutant M446G of phenylacetone monooxygenase of Thermobifida fusca, reaction is performed in presence of 10 U glucose-6-phosphate dehydrogenase and glucose-6-phosphate to recover NADPH | Thermobifida fusca | 3-isochromanone + NADP+ + H2O | - |
? | |
6-methoxy-1-indanone + NADPH + O2 | substrate was only accepted by phenylacetone monooxygenase of Pseudomonas fluorescens but not of Thermobifida fusca, reaction was performed in presence of 10 U glucose-6-phosphate dehydrogenase and glucose-6-phosphate to recover NADPH | Pseudomonas fluorescens | ? + NADP+ + H2O | - |
? | |
benzocyclobutanone + NADPH + O2 | reaction is performed in presence of 10 U glucose-6-phosphate dehydrogenase and glucose-6-phosphate to recover NADPH | Thermobifida fusca | 2-coumaranone + NADP+ + H2O | - |
? | |
benzocyclobutanone + NADPH + O2 | reaction was performed in presence of 10 U glucose-6-phosphate dehydrogenase and glucose-6-phosphate to recover NADPH | Pseudomonas fluorescens | 2-coumaranone + NADP+ + H2O | - |
? | |
phenylacetone + NADPH + O2 | reaction is performed in presence of 10 U glucose-6-phosphate dehydrogenase and glucose-6-phosphate to recover NADPH | Thermobifida fusca | benzyl acetate + NADP+ + H2O | - |
? | |
phenylacetone + NADPH + O2 | reaction was performed in presence of 10 U glucose-6-phosphate dehydrogenase and glucose-6-phosphate to recover NADPH | Pseudomonas fluorescens | benzyl acetate + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
4-hydroxyacetophenone monooxygenase | - |
Pseudomonas fluorescens |
Baeyer-Villiger monooxygenase | - |
Thermobifida fusca |
BVMO | - |
Thermobifida fusca |
HAPMO | - |
Pseudomonas fluorescens |
M-PAMO | mutant M446G of phenylacetone monooxygenase, phenylacetone monooxygenase mutein | Thermobifida fusca |
PAMO | - |
Thermobifida fusca |
phenylacetone monooxygenase | - |
Thermobifida fusca |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
enzyme activity is high from pH 9 to pH 10.5 | Thermobifida fusca |
9 | - |
enzyme activity is highest from pH 9 to pH 10.5 | Pseudomonas fluorescens |