Application | Comment | Organism |
---|---|---|
synthesis | the enzyme has catalytic potential as a biocatalyst | Thermobifida fusca |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics | Thermobifida fusca |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phenylacetone + NADPH + H+ + O2 | Thermobifida fusca | - |
benzyl acetate + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermobifida fusca | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
substrate specificity, diverse substrates, overview | Thermobifida fusca |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-1-acetoxy-phenylacetone + NADPH + O2 | - |
Thermobifida fusca | (R)-1-hydroxy-1-phenylacetone + NADP+ + H2O | - |
? | |
(S)-nicotine + NADPH + O2 | - |
Thermobifida fusca | ? | - |
? | |
3-phenylpenta-2,4-dione + NADPH + O2 | - |
Thermobifida fusca | (R)-phenylacetylcarbinol + NADP+ + H2O | the product is a well-known precursor in the synthesis of ephedrine and pseudoephedrine | ? | |
benzylacetone + NADPH + O2 | low activity | Thermobifida fusca | ? | - |
? | |
diketone + NADPH + O2 | - |
Thermobifida fusca | (R)-1-acetoxy-phenylacetone + NADP+ + H2O | - |
? | |
additional information | the recombinant His-tagged enzyme is active with a large range of sulfides and ketones, as well as with several sulfoxides, an amine and an organoboron compound, high enantioselectivity dependeing on the substrate, substrate specificity, overview | Thermobifida fusca | ? | - |
? | |
N,N-dimethylbenzylamine + NADPH + O2 | - |
Thermobifida fusca | N,N-dimethylbenzylamine N-oxide + NADP+ + H2O | - |
? | |
phenylacetone + NADPH + H+ + O2 | - |
Thermobifida fusca | benzyl acetate + NADP+ + H2O | - |
? | |
phenylboronic acid + NADPH + O2 | formation of phenol | Thermobifida fusca | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the Baeyer-Villiger monooxygenases | Thermobifida fusca |
PAMO | - |
Thermobifida fusca |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Thermobifida fusca |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | all substrates show a turnover between 1.2 s-1 and 3.6 s-1 | Thermobifida fusca |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Thermobifida fusca |