Any feedback?
Literature summary for 1.14.13.9 extracted from
- Heterologous expression and purification of kynurenine-3-monooxygenase from Pseudomonas fluorescens strain 17400 (2007), Protein Expr. Purif., 51, 324-333.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | the enzyme is an attractive target for the treatment of ischemic stroke | Pseudomonas fluorescens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| functional expression in Escherichia coli strain BL21 (DE3) | Pseudomonas fluorescens |
General Stability
| General Stability | Organism |
|---|---|
| disulfide reductants like dithiothreitol or 2-mercaptoethanol stabilize the enzyme | Pseudomonas fluorescens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-nitrobenzoylalanine | - |
Pseudomonas fluorescens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | steady-state kinetics and ligand perturbation of flavin fluorescence, overview | Pseudomonas fluorescens | |
| 0.0085 | - |
NADPH | recombinant enzyme, pH 7.5, 5°C | Pseudomonas fluorescens | |
| 0.012 | - |
kynurenine | recombinant enzyme, pH 7.5, 5°C | Pseudomonas fluorescens | |
| 0.034 | - |
O2 | recombinant enzyme, pH 7.5, 5°C | Pseudomonas fluorescens | |
| 0.053 | - |
kynurenine | recombinant enzyme, pH 7.5, 25°C | Pseudomonas fluorescens | |
| 0.071 | - |
O2 | recombinant enzyme, pH 7.5, 25°C | Pseudomonas fluorescens | |
| 0.091 | - |
NADPH | recombinant enzyme, pH 7.5, 25°C | Pseudomonas fluorescens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
x * 50000, recombinant enzyme, SDS-PAGE | Pseudomonas fluorescens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| kynurenine + NADPH + O2 | Pseudomonas fluorescens | the reaction is central to the tryptophan degradative pathway, overview, and takes place within microglial cells defining cellular concentrations of the N-methyl-D-aspatate receptor agonist quinolinate and antagonist kynurenate | 3-hydroxy-kynurenine + NADP+ + H2O | the product acts as apoptotic signal | ir | |
| kynurenine + NADPH + O2 | Pseudomonas fluorescens 17400 | the reaction is central to the tryptophan degradative pathway, overview, and takes place within microglial cells defining cellular concentrations of the N-methyl-D-aspatate receptor agonist quinolinate and antagonist kynurenate | 3-hydroxy-kynurenine + NADP+ + H2O | the product acts as apoptotic signal | ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | - |
- |
- |
| Pseudomonas fluorescens 17400 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme 8.8fold from Escherichia coli strain by ammonium sulfate fractionation and ion exchange chromatography to homogeneity | Pseudomonas fluorescens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| microglia | - |
Pseudomonas fluorescens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.15 | - |
purified recombinant enzyme | Pseudomonas fluorescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| kynurenine + NADPH + O2 | the reaction is central to the tryptophan degradative pathway, overview, and takes place within microglial cells defining cellular concentrations of the N-methyl-D-aspatate receptor agonist quinolinate and antagonist kynurenate | Pseudomonas fluorescens | 3-hydroxy-kynurenine + NADP+ + H2O | the product acts as apoptotic signal | ir | |
| kynurenine + NADPH + O2 | enzyme activity depends on the reduction state of the enzyme | Pseudomonas fluorescens | 3-hydroxy-kynurenine + NADP+ + H2O | - |
ir | |
| kynurenine + NADPH + O2 | the reaction is central to the tryptophan degradative pathway, overview, and takes place within microglial cells defining cellular concentrations of the N-methyl-D-aspatate receptor agonist quinolinate and antagonist kynurenate | Pseudomonas fluorescens 17400 | 3-hydroxy-kynurenine + NADP+ + H2O | the product acts as apoptotic signal | ir | |
| kynurenine + NADPH + O2 | enzyme activity depends on the reduction state of the enzyme | Pseudomonas fluorescens 17400 | 3-hydroxy-kynurenine + NADP+ + H2O | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50000, recombinant enzyme, SDS-PAGE | Pseudomonas fluorescens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| KMO | - |
Pseudomonas fluorescens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Pseudomonas fluorescens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.3 | - |
kynurenine | recombinant enzyme, pH 7.5, 5°C | Pseudomonas fluorescens | |
| 4.9 | - |
kynurenine | recombinant enzyme, pH 7.5, 25°C | Pseudomonas fluorescens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Pseudomonas fluorescens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 9.7 | - |
Pseudomonas fluorescens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | purified recombinant enzyme, 4°C, stable | Pseudomonas fluorescens |
| 8 | - |
purified recombinant enzyme, 4°C, 10 h, loss of 21% of activity | Pseudomonas fluorescens |
| 9 | - |
purified recombinant enzyme, 4°C, 10 h, loss of 32% of activity | Pseudomonas fluorescens |
| 10 | - |
purified recombinant enzyme, 4°C, 3 h, complete inactivation | Pseudomonas fluorescens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | flavoprotein | Pseudomonas fluorescens | |
| NADPH | dependent on | Pseudomonas fluorescens | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
