Cloned (Comment) | Organism |
---|---|
gene kh, DNA and amino acid sequence analysis of wild-type and mutant genes, expression in Spodoptera frugiperda Sf9 cells as His-tagged, soluble protein via the baculovirus infection system | Aedes aegypti |
Protein Variants | Comment | Organism |
---|---|---|
additional information | an inactive mutant lacks 162 nucleotides near the 3'-end of the mutant allele, the in-frame deletion results in loss of 54 amino acids leading to loss of enzyme activity and white eyes | Aedes aegypti |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chloride | mixed-type inhibition | Aedes aegypti | |
pyridoxal 5'-phosphate | noncompetitive | Aedes aegypti |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Aedes aegypti | |
0.82 | - |
NADPH | recombinant enzyme, pH 7.5, 37°C | Aedes aegypti | |
0.89 | - |
L-kynurenine | recombinant enzyme, pH 7.5, 37°C | Aedes aegypti | |
5.17 | - |
NADH | recombinant enzyme, pH 7.5, 37°C | Aedes aegypti |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | enzyme is hydrophobis and contains 2 transmembrane segments | Aedes aegypti | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
x * 54000, recombinant soluble enzyme not counting the His-tag, SDS-PAGE | Aedes aegypti |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-kynurenine + NADPH + O2 | Aedes aegypti | enzyme has a key role in L-tryptophan catabolism and in synthesis of ommochrome pigments in the eyes of the mosquitos | 3-hydroxy-L-kynurenine + NADP+ + H2O | - |
? | |
L-kynurenine + NADPH + O2 | Aedes aegypti Liverpool | enzyme has a key role in L-tryptophan catabolism and in synthesis of ommochrome pigments in the eyes of the mosquitos | 3-hydroxy-L-kynurenine + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aedes aegypti | Q86PM2 | yellow fever mosquito, black-eyed Liverpool strain | - |
Aedes aegypti Liverpool | Q86PM2 | yellow fever mosquito, black-eyed Liverpool strain | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from soluble fraction of Sf9 insect cells by nickel affinity chromatography | Aedes aegypti |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-kynurenine + NADH + H+ + O2 | - |
Aedes aegypti | 3-hydroxy-L-kynurenine + NAD+ + H2O | - |
? | |
L-kynurenine + NADH + H+ + O2 | - |
Aedes aegypti Liverpool | 3-hydroxy-L-kynurenine + NAD+ + H2O | - |
? | |
L-kynurenine + NADPH + O2 | - |
Aedes aegypti | 3-hydroxy-L-kynurenine + NADP+ + H2O | - |
? | |
L-kynurenine + NADPH + O2 | enzyme has a key role in L-tryptophan catabolism and in synthesis of ommochrome pigments in the eyes of the mosquitos | Aedes aegypti | 3-hydroxy-L-kynurenine + NADP+ + H2O | - |
? | |
L-kynurenine + NADPH + O2 | - |
Aedes aegypti Liverpool | 3-hydroxy-L-kynurenine + NADP+ + H2O | - |
? | |
L-kynurenine + NADPH + O2 | enzyme has a key role in L-tryptophan catabolism and in synthesis of ommochrome pigments in the eyes of the mosquitos | Aedes aegypti Liverpool | 3-hydroxy-L-kynurenine + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54000, recombinant soluble enzyme not counting the His-tag, SDS-PAGE | Aedes aegypti |
Synonyms | Comment | Organism |
---|---|---|
KMO | - |
Aedes aegypti |
kynurenine hydroxylase | - |
Aedes aegypti |
kynurenine monooxygenase | - |
Aedes aegypti |
More | the enzyme is a member of the glutathione reductase structural family | Aedes aegypti |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Aedes aegypti |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.85 | - |
NADH | recombinant enzyme, pH 7.5, 37°C | Aedes aegypti | |
1.88 | - |
L-kynurenine | recombinant enzyme, pH 7.5, 37°C | Aedes aegypti | |
2.03 | - |
NADPH | recombinant enzyme, pH 7.5, 37°C | Aedes aegypti |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Aedes aegypti |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | consensus domain sequence, probably FAD-containing | Aedes aegypti | |
NADH | low activity, ineffective cofactor | Aedes aegypti | |
NADPH | highly preferred cofactor with respect to NADH | Aedes aegypti |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
pyridoxal 5'-phosphate | recombinant enzyme, with respect to NADPH, pH 7.5, 37°C | Aedes aegypti | |
0.27 | - |
pyridoxal 5'-phosphate | recombinant enzyme, with respect to L-kynurenine, pH 7.5, 37°C | Aedes aegypti | |
11.2 | - |
chloride | recombinant enzyme, with respect to L-kynurenine, pH 7.5, 37°C | Aedes aegypti | |
24.5 | - |
chloride | recombinant enzyme, with respect to NADPH, pH 7.5, 37°C | Aedes aegypti |