Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADH | above 3 mM | Saccharomyces cerevisiae | |
p-chloromercuribenzoate | weak, 50% inhibition at 0.4 M | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
NADPH | under aerobic conditions | Saccharomyces cerevisiae | |
0.017 | - |
NADPH | under unaerobic conditions | Saccharomyces cerevisiae | |
0.04 | - |
L-kynurenine | under aerobic conditions | Saccharomyces cerevisiae | |
0.045 | - |
L-kynurenine | under unaerobic conditions | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-kynurenine + NADPH + O2 | Saccharomyces cerevisiae | rate limiting step in the pyridine nucleotide biosynthesis from tryptophan | ? + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
mutant E105 has an enzymatic defect of kynurenine 3-hydroxylase | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme activity with different cofactors and under anaerobic conditions | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-kynurenine + NADPH + O2 | - |
Saccharomyces cerevisiae | 3-hydroxy-L-kynurenine + NADP+ + H2O | - |
? | |
L-kynurenine + NADPH + O2 | rate limiting step in the pyridine nucleotide biosynthesis from tryptophan | Saccharomyces cerevisiae | ? + NADP+ | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.1 | 8.9 | about 50% of maximal activity at pH 7.1 and 8.9 | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | less effective than NADPH | Saccharomyces cerevisiae | |
NADPH | - |
Saccharomyces cerevisiae |