BRENDA - Enzyme Database
show all sequences of 1.14.13.89

Cloning, expression and purification of isoflavone-2'-hydroxylase from Astragalus membranaceus Bge. Var. mongolicus (Bge.) Hsiao

Chen, J.; Yuan, H.; Zhang, L.; Pan, H.; Xu, R.; Zhong, Y.; Chen, J.; Nan, P.; Protein Expr. Purif. 107, 83-89 (2015)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene AmI2'H or CYP81E42, DNA and amino acid sequence determination and analysis, recombinant expression of truncated enzyme, lacking the N-terminal membrane binding motif, in Escherichia coli strain BL21(DE3). Expression of the enzyme without removal of the transmembrane domain or insertion of the hydrophilic peptide AKKTSSKGKL is unsuccessful
Astragalus membranaceus
Engineering
Protein Variants
Commentary
Organism
additional information
modification of the gene sequence to facilitate protein expression and increase protein solubility, removal of the N-terminal membrane binding motif to enhance protein solubility
Astragalus membranaceus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
transmembrane enzyme
Astragalus membranaceus
16020
-
Organism
Organism
UniProt
Commentary
Textmining
Astragalus membranaceus
W5S082
var. mongolicus
-
Purification (Commentary)
Purification (Commentary)
Organism
recombinant truncated enzyme, lacking the N-terminal membrane binding motif, from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Astragalus membranaceus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
AmI2'H is expressed throughout the entire plant
Astragalus membranaceus
-
Subunits
Subunits
Commentary
Organism
More
secondary structure analysis of AmI2'H
Astragalus membranaceus
Synonyms
Synonyms
Commentary
Organism
AmI2'H
-
Astragalus membranaceus
CYP81E42
-
Astragalus membranaceus
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Astragalus membranaceus
Cloned(Commentary) (protein specific)
Commentary
Organism
gene AmI2'H or CYP81E42, DNA and amino acid sequence determination and analysis, recombinant expression of truncated enzyme, lacking the N-terminal membrane binding motif, in Escherichia coli strain BL21(DE3). Expression of the enzyme without removal of the transmembrane domain or insertion of the hydrophilic peptide AKKTSSKGKL is unsuccessful
Astragalus membranaceus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Astragalus membranaceus
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
modification of the gene sequence to facilitate protein expression and increase protein solubility, removal of the N-terminal membrane binding motif to enhance protein solubility
Astragalus membranaceus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
transmembrane enzyme
Astragalus membranaceus
16020
-
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant truncated enzyme, lacking the N-terminal membrane binding motif, from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Astragalus membranaceus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
AmI2'H is expressed throughout the entire plant
Astragalus membranaceus
-
Subunits (protein specific)
Subunits
Commentary
Organism
More
secondary structure analysis of AmI2'H
Astragalus membranaceus
General Information
General Information
Commentary
Organism
metabolism
the enzyme is involved in isoflavonoid biosynthesis
Astragalus membranaceus
physiological function
the enzyme promotes the accumulation of medicarpin malonyl glucoside
Astragalus membranaceus
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is involved in isoflavonoid biosynthesis
Astragalus membranaceus
physiological function
the enzyme promotes the accumulation of medicarpin malonyl glucoside
Astragalus membranaceus
Other publictions for EC 1.14.13.89
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746010
Uchida
-
Functional expression of cyto ...
Glycine max
Plant Biotechnol.
32
205-213
2015
1
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1
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-
-
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4
-
2
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8
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7
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4
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2
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2
6
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4
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8
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746367
Chen
Cloning, expression and purif ...
Astragalus membranaceus
Protein Expr. Purif.
107
83-89
2015
-
-
1
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1
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1
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1
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1
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1
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1
2
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1
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1
1
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1
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1
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1
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1
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-
-
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
745618
Gholampour
-
Identification of genes expre ...
Citrus x paradisi
J. Phytopathol.
162
811-819
2014
-
-
-
-
-
-
-
-
-
-
-
-
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1
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1
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1
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