BRENDA - Reference to 1.14.13.83; Id = 671939

Fe2+

the enzyme contains both a non-haem iron and an Fe-S centre

Rhodobacter capsulatus

precorrin-3A + NADH + O2

Rhodobacter capsulatus

the enzyme is involved in ring contraction, whereby an integral carbon atom of the tetrapyrrole-derived macrocycle is removed, and cobalt chelation during aerobic synthesis of vitamin B12, the catalytic cycle of CobZ, overview

precorrin-3B + NAD+ + H2O

-

?

Rhodobacter capsulatus

-

in bacteria such as Rhodobacter capsulatus CobG is substituted by an isofunctional protein called CobZ

-

precorrin-3A + NADH + H+ + O2 = precorrin-3B + NAD+ + H2O

reaction mechanism of CobZ

Rhodobacter capsulatus

precorrin-3A + NADH + O2

the enzyme is involved in ring contraction, whereby an integral carbon atom of the tetrapyrrole-derived macrocycle is removed, and cobalt chelation during aerobic synthesis of vitamin B12, the catalytic cycle of CobZ, overview

671939

Rhodobacter capsulatus

precorrin-3B + NAD+ + H2O

-

?

precorrin-3A + NADH + O2

the enzyme generates a hydroxy lactone intermediate

671939

Rhodobacter capsulatus

precorrin-3B + NAD+ + H2O

-

?

CobZ

-

Rhodobacter capsulatus

flavin

enzyme-bound

Rhodobacter capsulatus

flavin

enzyme-bound

Rhodobacter capsulatus

Fe2+

the enzyme contains both a non-haem iron and an Fe-S centre

Rhodobacter capsulatus

precorrin-3A + NADH + O2

Rhodobacter capsulatus

the enzyme is involved in ring contraction, whereby an integral carbon atom of the tetrapyrrole-derived macrocycle is removed, and cobalt chelation during aerobic synthesis of vitamin B12, the catalytic cycle of CobZ, overview

precorrin-3B + NAD+ + H2O

-

?

precorrin-3A + NADH + O2

the enzyme is involved in ring contraction, whereby an integral carbon atom of the tetrapyrrole-derived macrocycle is removed, and cobalt chelation during aerobic synthesis of vitamin B12, the catalytic cycle of CobZ, overview

671939

Rhodobacter capsulatus

precorrin-3B + NAD+ + H2O

-

?

precorrin-3A + NADH + O2

the enzyme generates a hydroxy lactone intermediate

671939

Rhodobacter capsulatus

precorrin-3B + NAD+ + H2O

-

?

698972

Schroeder

Demonstration that CobG, the m ...

Brucella melitensis 16M, Brucella melitensis, Pseudomonas denitrificans (nomen rejiciendum)

J. Biol. Chem.

284

4796-4805

2009

-

2

-

14

-

2

3

-

3

-

2

-

3

-

5

-

2

-

14

-

2

3

-

2

-

3

-

2

-

686660

Iida

Mechanism of the ring contract ...

Pseudomonas denitrificans (nomen rejiciendum)

FEBS J.

274

3475-3481

2007

-

2

-

1

-

3

-

1

-

1

-

2

-

3

-

671939

Heldt

Aerobic synthesis of vitamin B ...

Rhodobacter capsulatus

Biochem. Soc. Trans.

33

815-819

2005

-

1

-

1

-

1

-

1

-

2

-

1

-

1

-

1

-

1

-

1

-

2

-

650818

Stamford

Biosynthesis of vitamin B12: t ...

Pseudomonas denitrificans (nomen rejiciendum), Pseudomonas denitrificans (nomen rejiciendum) G3575

Chem. Biol.

4

445-451

1997

-

1

-

1

2

-

2

-

1

-

4

-

1

-

1

-

1

2

-

1

-

4

-

3209

Roessner

Overexpression in Escherichia ...

Pseudomonas denitrificans (nomen rejiciendum)

Protein Expr. Purif.

6

155-163

1995

-

1

-

1

2

1

-

1

-

1

-

2

-

2

-

1

-

1

-

1

2

1

-

1

-

2

-

15269

Scott

Biosynthesis of vitamin B12. D ...

Pseudomonas denitrificans (nomen rejiciendum)

FEBS Lett.

331

105-108

1993

-

1

-

2

1

-

1

-

2

-

1

-

2

1

-

2

-

15270

Debussche

Biosynthesis of the corrin mac ...

Pseudomonas denitrificans (nomen rejiciendum), Pseudomonas denitrificans (nomen rejiciendum) SC510 (RifT)

J. Bacteriol.

175

7430-7440

1993

-

1

-

1

-

1

-

4

-

2

-

1

-

1

-

6

-

1

-

1

-

1

-

1

-

4

-

1

-

1

-

6

-

Aerobic synthesis of vitamin B12: ring contraction and cobalt chelation

Data extracted from this reference: