Data extracted from this reference:
Metals/Ions
Fe2+
the enzyme contains both a non-haem iron and an Fe-S centre
Rhodobacter capsulatus
Natural Substrates/ Products (Substrates)
precorrin-3A + NADH + O2
Rhodobacter capsulatus
the enzyme is involved in ring contraction, whereby an integral carbon atom of the tetrapyrrole-derived macrocycle is removed, and cobalt chelation during aerobic synthesis of vitamin B12, the catalytic cycle of CobZ, overview
precorrin-3B + NAD+ + H2O
?
Organism
Rhodobacter capsulatus
in bacteria such as Rhodobacter capsulatus CobG is substituted by an isofunctional protein called CobZ
Reaction
precorrin-3A + NADH + H+ + O2 = precorrin-3B + NAD+ + H2O
reaction mechanism of CobZ
Rhodobacter capsulatus
Substrates and Products (Substrate)
precorrin-3A + NADH + O2
the enzyme is involved in ring contraction, whereby an integral carbon atom of the tetrapyrrole-derived macrocycle is removed, and cobalt chelation during aerobic synthesis of vitamin B12, the catalytic cycle of CobZ, overview
671939
Rhodobacter capsulatus
precorrin-3B + NAD+ + H2O
?
precorrin-3A + NADH + O2
the enzyme generates a hydroxy lactone intermediate
671939
Rhodobacter capsulatus
precorrin-3B + NAD+ + H2O
?
Synonyms
CobZ
Rhodobacter capsulatus
Cofactor
flavin
enzyme-bound
Rhodobacter capsulatus
Cofactor (protein specific)
flavin
enzyme-bound
Rhodobacter capsulatus
Metals/Ions (protein specific)
Fe2+
the enzyme contains both a non-haem iron and an Fe-S centre
Rhodobacter capsulatus
Natural Substrates/ Products (Substrates) (protein specific)
precorrin-3A + NADH + O2
Rhodobacter capsulatus
the enzyme is involved in ring contraction, whereby an integral carbon atom of the tetrapyrrole-derived macrocycle is removed, and cobalt chelation during aerobic synthesis of vitamin B12, the catalytic cycle of CobZ, overview
precorrin-3B + NAD+ + H2O
?
Substrates and Products (Substrate) (protein specific)
precorrin-3A + NADH + O2
the enzyme is involved in ring contraction, whereby an integral carbon atom of the tetrapyrrole-derived macrocycle is removed, and cobalt chelation during aerobic synthesis of vitamin B12, the catalytic cycle of CobZ, overview
671939
Rhodobacter capsulatus
precorrin-3B + NAD+ + H2O
?
precorrin-3A + NADH + O2
the enzyme generates a hydroxy lactone intermediate
671939
Rhodobacter capsulatus
precorrin-3B + NAD+ + H2O
?
Other publictions for EC 1.14.13.83
698972
Schroeder
Demonstration that CobG, the m ...
Brucella melitensis 16M, Brucella melitensis, Pseudomonas denitrificans (nomen rejiciendum)
J. Biol. Chem.
284
4796-4805
2009
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2
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14
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2
2
3
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3
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2
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3
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5
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2
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14
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3
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2
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3
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2
2
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686660
Iida
Mechanism of the ring contract ...
Pseudomonas denitrificans (nomen rejiciendum)
FEBS J.
274
3475-3481
2007
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2
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1
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1
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671939
Heldt
Aerobic synthesis of vitamin B ...
Rhodobacter capsulatus
Biochem. Soc. Trans.
33
815-819
2005
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1
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650818
Stamford
Biosynthesis of vitamin B12: t ...
Pseudomonas denitrificans (nomen rejiciendum), Pseudomonas denitrificans (nomen rejiciendum) G3575
Chem. Biol.
4
445-451
1997
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1
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1
1
2
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2
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4
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4
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3209
Roessner
Overexpression in Escherichia ...
Pseudomonas denitrificans (nomen rejiciendum)
Protein Expr. Purif.
6
155-163
1995
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1
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2
1
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15269
Scott
Biosynthesis of vitamin B12. D ...
Pseudomonas denitrificans (nomen rejiciendum)
FEBS Lett.
331
105-108
1993
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1
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1
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1
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15270
Debussche
Biosynthesis of the corrin mac ...
Pseudomonas denitrificans (nomen rejiciendum), Pseudomonas denitrificans (nomen rejiciendum) SC510 (RifT)
J. Bacteriol.
175
7430-7440
1993
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1
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1
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1
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4
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2
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1
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1
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6
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1
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1
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1
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1
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1
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1
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6
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