BRENDA - Enzyme Database
show all sequences of 1.14.13.83

Overexpression in Escherichia coli of 12 vitamin B12 biosynthetic enzymes

Roessner, C.A.; Spencer, J.B.; Ozaki, S.; Min, C.; Atshaves, B.P.; Nayar, P.; Anousis, N.; Stolowich, N.J.; Holderman, M.T.; Scott, A.I.; Protein Expr. Purif. 6, 155-163 (1995)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
overexpressed in recombinant strains of Escherichia coli
Pseudomonas denitrificans (nomen rejiciendum)
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
contains Fe4S4
Pseudomonas denitrificans (nomen rejiciendum)
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
46690
-
calculated from the gene sequence
Pseudomonas denitrificans (nomen rejiciendum)
48000
-
SDS-PAGE
Pseudomonas denitrificans (nomen rejiciendum)
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Pseudomonas denitrificans (nomen rejiciendum)
biosynthesis of vitamin B12
?
-
Pseudomonas denitrificans (nomen rejiciendum)
?
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas denitrificans (nomen rejiciendum)
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Pseudomonas denitrificans (nomen rejiciendum)
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
biosynthesis of vitamin B12
3209
Pseudomonas denitrificans (nomen rejiciendum)
?
-
3209
Pseudomonas denitrificans (nomen rejiciendum)
?
precorrin-3 + NADH + H+ + O2
-
3209
Pseudomonas denitrificans (nomen rejiciendum)
precorrin-3x + NAD+ + H2O
-
3209
Pseudomonas denitrificans (nomen rejiciendum)
ir
Synonyms
Synonyms
Commentary
Organism
CobG
-
Pseudomonas denitrificans (nomen rejiciendum)
precorrin-3X synthase
-
Pseudomonas denitrificans (nomen rejiciendum)
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Pseudomonas denitrificans (nomen rejiciendum)
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpressed in recombinant strains of Escherichia coli
Pseudomonas denitrificans (nomen rejiciendum)
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Pseudomonas denitrificans (nomen rejiciendum)
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
contains Fe4S4
Pseudomonas denitrificans (nomen rejiciendum)
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
46690
-
calculated from the gene sequence
Pseudomonas denitrificans (nomen rejiciendum)
48000
-
SDS-PAGE
Pseudomonas denitrificans (nomen rejiciendum)
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Pseudomonas denitrificans (nomen rejiciendum)
biosynthesis of vitamin B12
?
-
Pseudomonas denitrificans (nomen rejiciendum)
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas denitrificans (nomen rejiciendum)
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
biosynthesis of vitamin B12
3209
Pseudomonas denitrificans (nomen rejiciendum)
?
-
3209
Pseudomonas denitrificans (nomen rejiciendum)
?
precorrin-3 + NADH + H+ + O2
-
3209
Pseudomonas denitrificans (nomen rejiciendum)
precorrin-3x + NAD+ + H2O
-
3209
Pseudomonas denitrificans (nomen rejiciendum)
ir
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
698972
Schroeder
Demonstration that CobG, the m ...
Brucella melitensis 16M, Brucella melitensis, Pseudomonas denitrificans (nomen rejiciendum)
J. Biol. Chem.
284
4796-4805
2009
-
-
2
-
14
-
-
-
-
2
2
3
-
3
-
-
2
-
-
-
-
-
3
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
14
-
-
-
-
-
-
2
2
3
-
-
-
2
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
686660
Iida
Mechanism of the ring contract ...
Pseudomonas denitrificans (nomen rejiciendum)
FEBS J.
274
3475-3481
2007
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671939
Heldt
Aerobic synthesis of vitamin B ...
Rhodobacter capsulatus
Biochem. Soc. Trans.
33
815-819
2005
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
650818
Stamford
Biosynthesis of vitamin B12: t ...
Pseudomonas denitrificans (nomen rejiciendum), Pseudomonas denitrificans (nomen rejiciendum) G3575
Chem. Biol.
4
445-451
1997
-
-
1
-
-
-
-
-
-
1
1
2
-
2
-
-
1
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
1
2
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3209
Roessner
Overexpression in Escherichia ...
Pseudomonas denitrificans (nomen rejiciendum)
Protein Expr. Purif.
6
155-163
1995
-
-
1
-
-
-
-
-
-
1
2
1
-
1
-
-
1
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
2
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
15269
Scott
Biosynthesis of vitamin B12. D ...
Pseudomonas denitrificans (nomen rejiciendum)
FEBS Lett.
331
105-108
1993
-
-
1
-
-
-
-
-
-
-
2
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
15270
Debussche
Biosynthesis of the corrin mac ...
Pseudomonas denitrificans (nomen rejiciendum), Pseudomonas denitrificans (nomen rejiciendum) SC510 (RifT)
J. Bacteriol.
175
7430-7440
1993
-
-
1
-
-
1
-
-
-
1
-
4
-
2
-
-
1
-
-
-
1
-
6
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
1
-
4
-
-
-
1
-
-
1
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-