BRENDA - Enzyme Database show
show all sequences of 1.14.13.81

Molecular characterization and primary functional analysis of PeMPEC, a magnesium-protoporphyrin IX monomethyl ester cyclase gene of bamboo (Phyllostachys edulis)

Yang, L.; Lou, Y.; Peng, Z.; Zhao, H.; Sun, H.; Gao, Z.; Plant Cell Rep. 34, 2001-2011 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene PeMPEC, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis, recombinant overexpression in Arabidopsis thaliana Col-0 using the Agrobacterium tumefaciens strain GV310 transformation method. Gene PeMPEC driven by the CAMV 35S promoter is transferred into the Crd1 mutant leading to functional complementation of the mutant, the chlorophyll concentration of sense plants is 22-50% higher than that of the Crd1 mutant, and 7-20% higher than that of wild-type Col-0
Phyllostachys edulis
Engineering
Amino acid exchange
Commentary
Organism
additional information
chlorophyll fluorescence analysis of Arabidopsis wild-type Col-0 and PeMPEC transgenic plants, overview
Phyllostachys edulis
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
additional information
the enzyme sequence contains a transit peptide
Phyllostachys edulis
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Phyllostachys edulis
A0A0K1L5Y4
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
transcription of PeMPEC occurs mainly in the leaf
Phyllostachys edulis
-
additional information
very low or no enzyme expression in root, stem, and sheath
Phyllostachys edulis
-
seedling
-
Phyllostachys edulis
-
Subunits
Subunits
Commentary
Organism
?
x * 47900, about, proenzyme, sequence calculation, x * 42700, about, mature enzyme, sequence calculation, x * 62000, recombinant tagged enzyme, SDS-PAGE
Phyllostachys edulis
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Phyllostachys edulis
sequence calculation
-
9.1
Cloned(Commentary) (protein specific)
Commentary
Organism
gene PeMPEC, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis, recombinant overexpression in Arabidopsis thaliana Col-0 using the Agrobacterium tumefaciens strain GV310 transformation method. Gene PeMPEC driven by the CAMV 35S promoter is transferred into the Crd1 mutant leading to functional complementation of the mutant, the chlorophyll concentration of sense plants is 22-50% higher than that of the Crd1 mutant, and 7-20% higher than that of wild-type Col-0
Phyllostachys edulis
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
chlorophyll fluorescence analysis of Arabidopsis wild-type Col-0 and PeMPEC transgenic plants, overview
Phyllostachys edulis
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
additional information
the enzyme sequence contains a transit peptide
Phyllostachys edulis
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
transcription of PeMPEC occurs mainly in the leaf
Phyllostachys edulis
-
additional information
very low or no enzyme expression in root, stem, and sheath
Phyllostachys edulis
-
seedling
-
Phyllostachys edulis
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 47900, about, proenzyme, sequence calculation, x * 42700, about, mature enzyme, sequence calculation, x * 62000, recombinant tagged enzyme, SDS-PAGE
Phyllostachys edulis
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Phyllostachys edulis
sequence calculation
-
9.1
Expression
Organism
Commentary
Expression
Phyllostachys edulis
temperature has a temporally sensitive and complex effect on PeMPEC transcription
additional information
General Information
General Information
Commentary
Organism
physiological function
MPEC is an essential enzyme in chlorophyll biosynthesis
Phyllostachys edulis
General Information (protein specific)
General Information
Commentary
Organism
physiological function
MPEC is an essential enzyme in chlorophyll biosynthesis
Phyllostachys edulis
Expression (protein specific)
Organism
Commentary
Expression
Phyllostachys edulis
temperature has a temporally sensitive and complex effect on PeMPEC transcription
additional information
Other publictions for EC 1.14.13.81
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746218
Wang
Mutation in Mg-protoporphyrin ...
Oryza sativa Japonica Group
PLoS ONE
12
e0171118
2017
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746223
Sheng
Yellow-Leaf 1 encodes a magne ...
Oryza sativa
PLoS ONE
12
e0177989
2017
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744998
Hollingshead
Synthesis of chlorophyll-bind ...
Synechocystis sp. PCC 6803
Front. Plant Sci.
7
292
2016
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746095
Kong
The catalytic subunit of magn ...
Oryza sativa
Plant Mol. Biol.
92
177-191
2016
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746054
Yang
Molecular characterization an ...
Phyllostachys edulis
Plant Cell Rep.
34
2001-2011
2015
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744039
Lakshmi
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Molecular cloning and sequenc ...
Saccharum arundinaceum
Ann. Biol.
30
15-20
2014
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744871
Bollivar
The Ycf54 protein is part of ...
Hordeum vulgare
FEBS J.
281
2377-2386
2014
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745807
Schlicke
Induced deactivation of genes ...
Arabidopsis thaliana
Mol. Plant
7
1211-1227
2014
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726735
Boldareva-Nuianzina
Distribution and origin of oxy ...
Acidiphilium multivorum, Acidiphilium multivorum AIU301, Agrobacterium albertimagni, Agrobacterium albertimagni AOL15, Ahrensia sp. R2A130, Bradyrhizobium sp., Bradyrhizobium sp. BTAi1, Bradyrhizobium sp. ORS278, Brevundimonas subvibrioides, Brevundimonas subvibrioides ATCC 15264, Citromicrobium bathyomarinum, Citromicrobium bathyomarinum JL354, Congregibacter litoralis, Congregibacter litoralis KT71, Dinoroseobacter shibae, Dinoroseobacter shibae DFL 12, Erythrobacter sp., Erythrobacter sp. NAP1, gammaproteobacteria, gammaproteobacteria HTCC2080, gammaproteobacteria NOR51-B, gammaproteobacteria NOR5-3, gamma proteobacterium HIMB55, Hoeflea phototrophica, Hoeflea phototrophica DFL-43, Jannaschia sp. CCS1, Labrenzia alexandrii, Labrenzia alexandrii DFL-11, Limnohabitans sp., Limnohabitans sp. Rim28, Limnohabitans sp. Rim47, Methylobacterium radiotolerans, Methylobacterium sp., Methylobacterium sp. 4-46, Methylocella silvestris, Methylocella silvestris BL2, Methylorubrum extorquens, Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1, Methylorubrum populi, Methylorubrum populi BJ001, Methyloversatilis universalis, Methyloversatilis universalis FAM5, Rhodobacter sp., Rhodobacter sphaeroides, Rhodobacter sp. SW2, Rhodomicrobium vannielii, Rhodomicrobium vannielii ATCC 17100, Rhodopseudomonas palustris, Rhodospirillum centenum, Roseobacter denitrificans, Roseobacter litoralis, Roseobacter litoralis Och 149c, Roseobacter sp., Roseobacter sp. AzwK-3b, Roseobacter sp. CCS2, Roseovarius sp. 217, Roseovarius sp., Roseovarius sp. TM1035, Rubrivivax benzoatilyticus, Rubrivivax benzoatilyticus JA2, Rubrivivax gelatinosus, Rubrivivax gelatinosus IL144, Sphingomonas sp., Yoonia vestfoldensis, Yoonia vestfoldensis SKA53
Appl. Environ. Microbiol.
79
2596-2604
2013
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727915
Hollingshead
Conserved chloroplast open-rea ...
Synechocystis sp.
J. Biol. Chem.
287
27823-27833
2012
1
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728517
Albus
LCAA, a novel factor required ...
Nicotiana tabacum
Plant Physiol.
160
1923-1939
2012
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698617
Tang
Role of the AcsF protein in Ch ...
Chloroflexus aurantiacus, Chloroflexus aurantiacus J-10-fl
J. Bacteriol.
191
3580-3587
2009
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687758
Minamizaki
Identification of two homologo ...
Synechocystis sp. PCC 6803
J. Biol. Chem.
283
2684-2692
2008
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700629
Liu
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MPEC: An important gene in the ...
Arabidopsis thaliana, Brassica napus, Chlamydomonas reinhardtii, Cucumis sativus, Hordeum vulgare, Ipomoea nil, Nicotiana tabacum, Oryza sativa, Rosa davurica, Rubrivivax gelatinosus, Salix babylonica, Spinacia oleracea, Trifolium repens, Triticum aestivum
Photosynthetica
46
321-328
2008
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700711
Bang
Role of Arabidopsis CHL27 prot ...
Arabidopsis thaliana
Plant Cell Physiol.
49
1350-1363
2008
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689645
Gough
A new method for isolating phy ...
Rhodobacter capsulatus
Plant Physiol. Biochem.
45
932-936
2007
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689775
Gray
Single-enzyme conversion of FM ...
Rhodospirillum rubrum
Proc. Natl. Acad. Sci. USA
104
2921-2926
2007
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676645
Gadjieva
Analysis of gun phenotype in b ...
Hordeum vulgare
Plant Physiol. Biochem.
43
901-908
2005
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676791
Rzeznicka
Xantha-l encodes a membrane su ...
Hordeum vulgare
Proc. Natl. Acad. Sci. USA
102
5886-5891
2005
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636329
Bollivar
The chlorophyll biosynthetic e ...
Chlamydomonas reinhardtii, Synechocystis sp.
Plant Physiol.
112
105-114
1996
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636332
Whyte
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Further observations on the ma ...
Cucumis sativus
Biochem. J.
290
355-359
1993
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636330
Walker
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Synthesis of divinyl protochlo ...
Cucumis sativus
Biochem. J.
276
691-697
1991
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636331
Walker
The magnesium-protoporphyrin I ...
Cucumis sativus
Biochem. J.
255
685-692
1988
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636335
Wong
Properties of the magnesium-pr ...
Cucumis sativus
Plant Physiol.
79
730-733
1985
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636333
Wong
Resolution and reconstitution ...
Cucumis sativus
Plant Physiol.
75
658-661
1984
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636334
Fuesler
Localization of magnesium-chel ...
Cucumis sativus
Plant Physiol.
75
662-664
1984
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