Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CN- | iron chelator strongly inhibits enzyme in reconstituted in vitro assay but not or only slightly in intact chloroplast systems with membrane-bound haemoprotein | Cucumis sativus | |
N3- | iron chelator strongly inhibits enzyme in reconstituted in vitro assay but not or only slightly in intact chloroplast systems with membrane-bound haemoprotein | Cucumis sativus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast envelope | - |
Arabidopsis thaliana | 9941 | - |
chloroplast membrane | - |
Hordeum vulgare | 31969 | - |
chloroplast membrane | - |
Chlamydomonas reinhardtii | 31969 | - |
chloroplast membrane | membrane bound cell extract from cucumbers | Cucumis sativus | 31969 | - |
cytoplasm | soluble cell extract from cucumbers | Cucumis sativus | 5737 | - |
additional information | recombination of chloroplast membrane and cytoplasm fractions is necessary to restore cucumber cyclase activity in vitro | Cucumis sativus | - |
- |
soluble | - |
Hordeum vulgare | - |
- |
thylakoid membrane | - |
Arabidopsis thaliana | 42651 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
soluble fraction, membrane bound component is more labile | Cucumis sativus |
43000 | - |
calculted from predicted 370 amino acid residue sequence, 1402 bp, PNZIP from Pharbitis nil | Ipomoea nil |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 | Nicotiana tabacum | - |
protochlorophyllide a + NADP+ + H2O | intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a | ? | |
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 | Cucumis sativus | - |
protochlorophyllide a + NADP+ + H2O | intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a | ? | |
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 | Oryza sativa | - |
protochlorophyllide a + NADP+ + H2O | intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a | ? | |
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 | Hordeum vulgare | - |
protochlorophyllide a + NADP+ + H2O | intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a | ? | |
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 | Rosa davurica | - |
protochlorophyllide a + NADP+ + H2O | intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a | ? | |
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 | Salix babylonica | - |
protochlorophyllide a + NADP+ + H2O | intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a | ? | |
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 | Spinacia oleracea | - |
protochlorophyllide a + NADP+ + H2O | intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a | ? | |
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 | Triticum aestivum | - |
protochlorophyllide a + NADP+ + H2O | intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a | ? | |
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 | Trifolium repens | - |
protochlorophyllide a + NADP+ + H2O | intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a | ? | |
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 | Arabidopsis thaliana | - |
protochlorophyllide a + NADP+ + H2O | intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a | ? | |
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 | Chlamydomonas reinhardtii | - |
protochlorophyllide a + NADP+ + H2O | intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a | ? | |
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 | Rubrivivax gelatinosus | - |
protochlorophyllide a + NADP+ + H2O | intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a | ? | |
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 | Ipomoea nil | - |
protochlorophyllide a + NADP+ + H2O | intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a | ? | |
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 | Brassica napus | - |
protochlorophyllide a + NADP+ + H2O | intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9M591 | - |
- |
Brassica napus | Q7XJI2 | - |
- |
Chlamydomonas reinhardtii | Q9AR22 | green alga | - |
Chlamydomonas reinhardtii | Q9LD46 | green alga | - |
Cucumis sativus | - |
cucumber | - |
Hordeum vulgare | Q5EFU4 | barley | - |
Ipomoea nil | Q40093 | first MPEC gene identification in cDNA library (PNZIP) | - |
Nicotiana tabacum | - |
tobacco | - |
Oryza sativa | - |
rice | - |
Rosa davurica | - |
- |
- |
Rubrivivax gelatinosus | P0DJN9 | purple bacterium | - |
Salix babylonica | Q7XJI4 | - |
- |
Spinacia oleracea | Q7XJI5 | spinach | - |
Trifolium repens | Q7XJI1 | - |
- |
Triticum aestivum | Q7XJI6 | wheat | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | - |
Cucumis sativus | - |
leaf | strongest expression | Nicotiana tabacum | - |
leaf | wheat | Triticum aestivum | - |
mesophyll | specifically expressed in photosynthetic active cells | Ipomoea nil | - |
additional information | in photosynthetic tissue, undetectable in non-photosynthetic tissues | Cucumis sativus | - |
additional information | in photosynthetic tissue, undetectable in non-photosynthetic tissues | Oryza sativa | - |
additional information | in photosynthetic tissue, undetectable in non-photosynthetic tissues | Hordeum vulgare | - |
additional information | in photosynthetic tissue, undetectable in non-photosynthetic tissues | Rosa davurica | - |
additional information | in photosynthetic tissue, undetectable in non-photosynthetic tissues | Salix babylonica | - |
additional information | in photosynthetic tissue, undetectable in non-photosynthetic tissues | Spinacia oleracea | - |
additional information | in photosynthetic tissue, undetectable in non-photosynthetic tissues | Triticum aestivum | - |
additional information | in photosynthetic tissue, undetectable in non-photosynthetic tissues | Trifolium repens | - |
additional information | in photosynthetic tissue, undetectable in non-photosynthetic tissues | Arabidopsis thaliana | - |
additional information | in photosynthetic tissue, undetectable in non-photosynthetic tissues | Chlamydomonas reinhardtii | - |
additional information | in photosynthetic tissue, undetectable in non-photosynthetic tissues | Rubrivivax gelatinosus | - |
additional information | in photosynthetic tissue, undetectable in non-photosynthetic tissues | Ipomoea nil | - |
additional information | in photosynthetic tissue, undetectable in non-photosynthetic tissues | Brassica napus | - |
additional information | no expression in root and flower, expression in photosynthetic tissue, undetectable in non-photosynthetic tissues | Nicotiana tabacum | - |
stem | weaker expression than in stem | Nicotiana tabacum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 | - |
Nicotiana tabacum | protochlorophyllide a + NADP+ + H2O | intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a | ? | |
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 | - |
Cucumis sativus | protochlorophyllide a + NADP+ + H2O | intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a | ? | |
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 | - |
Oryza sativa | protochlorophyllide a + NADP+ + H2O | intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a | ? | |
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 | - |
Hordeum vulgare | protochlorophyllide a + NADP+ + H2O | intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a | ? | |
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 | - |
Rosa davurica | protochlorophyllide a + NADP+ + H2O | intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a | ? | |
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 | - |
Salix babylonica | protochlorophyllide a + NADP+ + H2O | intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a | ? | |
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 | - |
Spinacia oleracea | protochlorophyllide a + NADP+ + H2O | intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a | ? | |
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 | - |
Triticum aestivum | protochlorophyllide a + NADP+ + H2O | intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a | ? | |
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 | - |
Trifolium repens | protochlorophyllide a + NADP+ + H2O | intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a | ? | |
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 | - |
Arabidopsis thaliana | protochlorophyllide a + NADP+ + H2O | intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a | ? | |
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 | - |
Chlamydomonas reinhardtii | protochlorophyllide a + NADP+ + H2O | intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a | ? | |
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 | - |
Rubrivivax gelatinosus | protochlorophyllide a + NADP+ + H2O | intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a | ? | |
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 | - |
Ipomoea nil | protochlorophyllide a + NADP+ + H2O | intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a | ? | |
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 | - |
Brassica napus | protochlorophyllide a + NADP+ + H2O | intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a | ? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 2 * ?, highly conserved leucin zipper domain and EXnEXRH motif (binding binuclear-iron cluster) | Nicotiana tabacum |
heterodimer | 2 * ?, highly conserved leucin zipper domain and EXnEXRH motif (binding binuclear-iron cluster) | Cucumis sativus |
heterodimer | 2 * ?, highly conserved leucin zipper domain and EXnEXRH motif (binding binuclear-iron cluster) | Chlamydomonas reinhardtii |
heterodimer | 2 * ?, highly conserved leucin zipper domain and EXnEXRH motif (binding binuclear-iron cluster) | Rubrivivax gelatinosus |
heterodimer | 2 * ?, highly conserved leucin zipper domain and EXnEXRH motif (binding binuclear-iron cluster) | Ipomoea nil |
heterodimer | 2 * ?, highly conserved leucin zipper domain and EXnEXRH motif (binding binuclear-iron cluster) | Brassica napus |
heterodimer | highly conserved leucin zipper domain and EXnEXRH motif (binding binuclear-iron cluster) | Oryza sativa |
heterodimer | highly conserved leucin zipper domain and EXnEXRH motif (binding binuclear-iron cluster) | Hordeum vulgare |
heterodimer | highly conserved leucin zipper domain and EXnEXRH motif (binding binuclear-iron cluster) | Rosa davurica |
heterodimer | highly conserved leucin zipper domain and EXnEXRH motif (binding binuclear-iron cluster) | Salix babylonica |
heterodimer | highly conserved leucin zipper domain and EXnEXRH motif (binding binuclear-iron cluster) | Spinacia oleracea |
heterodimer | highly conserved leucin zipper domain and EXnEXRH motif (binding binuclear-iron cluster) | Triticum aestivum |
heterodimer | highly conserved leucin zipper domain and EXnEXRH motif (binding binuclear-iron cluster) | Trifolium repens |
heterodimer | highly conserved leucin zipper domain and EXnEXRH motif (binding binuclear-iron cluster) | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
AcsF protein | previously named orf358 | Rubrivivax gelatinosus |
ATZIP protein | - |
Arabidopsis thaliana |
BNZIP protein | - |
Brassica napus |
CHL27 protein | - |
Arabidopsis thaliana |
CHL27 protein | Arabidopsis thaliana | Arabidopsis thaliana |
CRD1 | - |
Chlamydomonas reinhardtii |
Crd1 protein | Chlamydomonas reinhardtii, first identified as gene in response to copper deficiency, one of two isozymes | Chlamydomonas reinhardtii |
CSZIP protein | - |
Cucumis sativus |
Cth1 protein | copper target homolog 1, isoenzyme to Crd1 | Chlamydomonas reinhardtii |
magnesium-protoporphyrin IX monomethyl ester cyclase | - |
Nicotiana tabacum |
magnesium-protoporphyrin IX monomethyl ester cyclase | - |
Cucumis sativus |
magnesium-protoporphyrin IX monomethyl ester cyclase | - |
Oryza sativa |
magnesium-protoporphyrin IX monomethyl ester cyclase | - |
Hordeum vulgare |
magnesium-protoporphyrin IX monomethyl ester cyclase | - |
Rosa davurica |
magnesium-protoporphyrin IX monomethyl ester cyclase | - |
Salix babylonica |
magnesium-protoporphyrin IX monomethyl ester cyclase | - |
Spinacia oleracea |
magnesium-protoporphyrin IX monomethyl ester cyclase | - |
Triticum aestivum |
magnesium-protoporphyrin IX monomethyl ester cyclase | - |
Trifolium repens |
magnesium-protoporphyrin IX monomethyl ester cyclase | - |
Arabidopsis thaliana |
magnesium-protoporphyrin IX monomethyl ester cyclase | - |
Chlamydomonas reinhardtii |
magnesium-protoporphyrin IX monomethyl ester cyclase | - |
Rubrivivax gelatinosus |
magnesium-protoporphyrin IX monomethyl ester cyclase | - |
Ipomoea nil |
magnesium-protoporphyrin IX monomethyl ester cyclase | - |
Brassica napus |
MgPME cyclase | - |
Nicotiana tabacum |
MgPME cyclase | - |
Cucumis sativus |
MgPME cyclase | - |
Oryza sativa |
MgPME cyclase | - |
Hordeum vulgare |
MgPME cyclase | - |
Rosa davurica |
MgPME cyclase | - |
Salix babylonica |
MgPME cyclase | - |
Spinacia oleracea |
MgPME cyclase | - |
Triticum aestivum |
MgPME cyclase | - |
Trifolium repens |
MgPME cyclase | - |
Arabidopsis thaliana |
MgPME cyclase | - |
Chlamydomonas reinhardtii |
MgPME cyclase | - |
Rubrivivax gelatinosus |
MgPME cyclase | - |
Ipomoea nil |
MgPME cyclase | - |
Brassica napus |
MPEC gene product | - |
Nicotiana tabacum |
MPEC gene product | - |
Cucumis sativus |
MPEC gene product | - |
Oryza sativa |
MPEC gene product | - |
Hordeum vulgare |
MPEC gene product | - |
Rosa davurica |
MPEC gene product | - |
Salix babylonica |
MPEC gene product | - |
Spinacia oleracea |
MPEC gene product | - |
Triticum aestivum |
MPEC gene product | - |
Trifolium repens |
MPEC gene product | - |
Arabidopsis thaliana |
MPEC gene product | - |
Chlamydomonas reinhardtii |
MPEC gene product | - |
Rubrivivax gelatinosus |
MPEC gene product | - |
Ipomoea nil |
MPEC gene product | - |
Brassica napus |
NTZIP protein | - |
Nicotiana tabacum |
OSZIP protein | - |
Oryza sativa |
PNZIP protein | - |
Ipomoea nil |
RDZIP protein | - |
Rosa davurica |
SBZIP protein | - |
Salix babylonica |
SOZIP protein | - |
Spinacia oleracea |
TAZIP protein | - |
Triticum aestivum |
TRZIP protein | - |
Trifolium repens |
Xantha-l | - |
Hordeum vulgare |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Nicotiana tabacum | |
NADPH | - |
Cucumis sativus | |
NADPH | - |
Oryza sativa | |
NADPH | - |
Hordeum vulgare | |
NADPH | - |
Rosa davurica | |
NADPH | - |
Salix babylonica | |
NADPH | - |
Spinacia oleracea | |
NADPH | - |
Triticum aestivum | |
NADPH | - |
Trifolium repens | |
NADPH | - |
Arabidopsis thaliana | |
NADPH | - |
Chlamydomonas reinhardtii | |
NADPH | - |
Rubrivivax gelatinosus | |
NADPH | - |
Ipomoea nil | |
NADPH | - |
Brassica napus |
Organism | Comment | Expression |
---|---|---|
Ipomoea nil | exposure to far-red radiation at the end of day or red radiation in the middle of night reduce PNZIP mRNA (phytochrome regulation in the dark) | down |
Nicotiana tabacum | strong irradiance | down |
Ipomoea nil | endogenous 24 h rhythm and regulation by phytochrome in the dark | additional information |
Nicotiana tabacum | radiant energy and low temperature | up |
General Information | Comment | Organism |
---|---|---|
malfunction | a Chl27-antisense Arabidopsis mutant accumulates the cyclase substrate magnesium-protoporphyrin IX monomethyl ester and does not produce protochlorophyllide a | Arabidopsis thaliana |
malfunction | a Rubrivivax gelatinosus strain with disrupted acsF gene cannot synthesize bacteriochlorophyll under oxygenated conditions but accumulates the substrate magnesium-protoporphyrin IX monomethyl ester, in contrast, under low-oxygene conditions the phenotype is similar to the wild-type, an alternative pathway for the reaction exists vie the bchE genes | Rubrivivax gelatinosus |
malfunction | crd1 strains fail to accumulate photosystem 1 (PS 1) and light-harvesting complex 1 (LHC 1) during hypoxia or copper deficiency, and have reduced amounts of LHC 2, Crd1 abundance is increased in copper or oxygen deficient cells | Chlamydomonas reinhardtii |
malfunction | Cth1 accumulates in copper-sufficient, oxygenated cells | Chlamydomonas reinhardtii |
malfunction | transgenic tobacco with antisense NTZIP-RNA displays chlorosis and a lack of the ability to turn green under normal growth conditions | Nicotiana tabacum |
malfunction | xantha-l35 and viridis-k deficient mutants grown in the dark, fed with delta-aminolevulinic acid, accumulate the substrate magnesium-protoporphyrin IX monomethyl ester and produce reduced amounts of protochlorophyllide a, in vitro complementation assay with mutants the aerobic cyclase is composed of at least 3 gene products, 1 soluble and 2 membrane-bound proteins (xantha-l and viridian-k) | Hordeum vulgare |
metabolism | protochlorophyllide a production (formation of the isocyclic ring) via several intermediates in the chlorophyll biosynthesis pathway | Nicotiana tabacum |
metabolism | protochlorophyllide a production (formation of the isocyclic ring) via several intermediates in the chlorophyll biosynthesis pathway | Cucumis sativus |
metabolism | protochlorophyllide a production (formation of the isocyclic ring) via several intermediates in the chlorophyll biosynthesis pathway | Oryza sativa |
metabolism | protochlorophyllide a production (formation of the isocyclic ring) via several intermediates in the chlorophyll biosynthesis pathway | Hordeum vulgare |
metabolism | protochlorophyllide a production (formation of the isocyclic ring) via several intermediates in the chlorophyll biosynthesis pathway | Rosa davurica |
metabolism | protochlorophyllide a production (formation of the isocyclic ring) via several intermediates in the chlorophyll biosynthesis pathway | Salix babylonica |
metabolism | protochlorophyllide a production (formation of the isocyclic ring) via several intermediates in the chlorophyll biosynthesis pathway | Spinacia oleracea |
metabolism | protochlorophyllide a production (formation of the isocyclic ring) via several intermediates in the chlorophyll biosynthesis pathway | Triticum aestivum |
metabolism | protochlorophyllide a production (formation of the isocyclic ring) via several intermediates in the chlorophyll biosynthesis pathway | Trifolium repens |
metabolism | protochlorophyllide a production (formation of the isocyclic ring) via several intermediates in the chlorophyll biosynthesis pathway | Arabidopsis thaliana |
metabolism | protochlorophyllide a production (formation of the isocyclic ring) via several intermediates in the chlorophyll biosynthesis pathway | Chlamydomonas reinhardtii |
metabolism | protochlorophyllide a production (formation of the isocyclic ring) via several intermediates in the chlorophyll biosynthesis pathway | Rubrivivax gelatinosus |
metabolism | protochlorophyllide a production (formation of the isocyclic ring) via several intermediates in the chlorophyll biosynthesis pathway | Ipomoea nil |
metabolism | protochlorophyllide a production (formation of the isocyclic ring) via several intermediates in the chlorophyll biosynthesis pathway | Brassica napus |