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Literature summary for 1.14.13.81 extracted from

  • Tang, K.H.; Wen, J.; Li, X.; Blankenship, R.E.
    Role of the AcsF protein in Chloroflexus aurantiacus (2009), J. Bacteriol., 191, 3580-3587.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.81

Cloned(Commentary)

Cloned (Comment) Organism
RNA is isolated to synthesize cDNA and quantitative real-time PCR is carried out for anaerobic and semiaerobic growth conditions Chloroflexus aurantiacus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane significant portion of the protein is not accessible to proteases, chlorosome envelop is a special lipid monolayer, complement of chlorophyll pigments, self-assembled into antenna structures Chloroflexus aurantiacus 16020
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Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 SDS-PAGE, trypsin-digested Chloroflexus aurantiacus
45000
-
 calculated from the acsF gene sequence encoding 375 amino acid residues Chloroflexus aurantiacus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
magnesium-protoporphyrin monomethyl ester + NADPH + H+ + O2 Chloroflexus aurantiacus bacteria are grown under anaerobic (dark green culture color) or semiaerobic (orange-red culture color) conditions and express the enzyme genes under both conditions to similar levels, though the enzyme is normally working under aerobic conditions magnesium-divinyl-protochlorophyllide a + NADP+ + H2O
-
 ?
magnesium-protoporphyrin monomethyl ester + NADPH + H+ + O2 Chloroflexus aurantiacus J-10-fl bacteria are grown under anaerobic (dark green culture color) or semiaerobic (orange-red culture color) conditions and express the enzyme genes under both conditions to similar levels, though the enzyme is normally working under aerobic conditions magnesium-divinyl-protochlorophyllide a + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Chloroflexus aurantiacus A9WIR9 green phototrophic bacterium
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Chloroflexus aurantiacus J-10-fl A9WIR9 green phototrophic bacterium
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Purification (Commentary)

Purification (Comment) Organism
cells are centrifuged, sonicated, cell debris removed by centrifugation, membrane fraction separated from the soluble fraction by ultracentrifugation, chlorosome fractioned by sucrose density gradient (15-45%), for SDS-PAGE chlorosome fraction is incubated in methanol, centrifuged, the pellet containing the chlorosome envelop is subjected to SDS-PAGE, protein identification by MALDI-TOF and liquid chromatography-tandem mass spectrometry Chloroflexus aurantiacus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
magnesium-protoporphyrin monomethyl ester + NADPH + H+ + O2 bacteria are grown under anaerobic (dark green culture color) or semiaerobic (orange-red culture color) conditions and express the enzyme genes under both conditions to similar levels, though the enzyme is normally working under aerobic conditions Chloroflexus aurantiacus magnesium-divinyl-protochlorophyllide a + NADP+ + H2O
-
 ?
magnesium-protoporphyrin monomethyl ester + NADPH + H+ + O2 bacteria are grown under anaerobic (dark green culture color) or semiaerobic (orange-red culture color) conditions and express the enzyme genes under both conditions to similar levels, though the enzyme is normally working under aerobic conditions Chloroflexus aurantiacus J-10-fl magnesium-divinyl-protochlorophyllide a + NADP+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
AcsF protein
-
 Chloroflexus aurantiacus
magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase
-
 Chloroflexus aurantiacus

Cofactor

Cofactor Comment Organism Structure
heme high probability for binuclear iron motif is proposed by sequence alignment, and a significant iron amount in the chlorosome fraction cannot be assigned to other chlorosome proteins Chloroflexus aurantiacus

Expression

Organism Comment Expression
Chloroflexus aurantiacus the aerobic magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase is expressed under semiaerobic and anaerobic conditions, the levels are similar under semiaerobic and anaerobic conditions additional information