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Literature summary for 1.14.13.8 extracted from

  • Motika, M.S.; Zhang, J.; Ralph, E.C.; Dwyer, M.A.; Cashman, J.R.
    pH dependence on functional activity of human and mouse flavin-containing monooxygenase 5 (2012), Biochem. Pharmacol., 83, 962-968.
    View publication on PubMed
Search for more literature for 1.14.13.8

Cloned(Commentary)

Cloned (Comment) Organism
expression as maltose-binding fusion proteins in Escherichia coli Homo sapiens
expression as maltose-binding fusion proteins in Escherichia coli Mus musculus

Protein Variants

Protein Variants Comment Organism
D227K pKa value 7.3 for N-oxygenation of 10-(N,N-dimethylaminooctyl)2-(trifluoromethyl)phenothiazene, compared with 6.9 for wild-type Homo sapiens
G182E pKa value 6.6 for N-oxygenation of 10-(N,N-dimethylaminooctyl)2-(trifluoromethyl)phenothiazene, compared with 6.9 for wild-type Homo sapiens
H228Y pKa value 6.6 for N-oxygenation of 10-(N,N-dimethylaminooctyl)2-(trifluoromethyl)phenothiazene, compared with 7.7 for wild-type Mus musculus
K227D pKa value 6.6 for N-oxygenation of 10-(N,N-dimethylaminooctyl)2-(trifluoromethyl)phenothiazene, compared with 7.7 for wild-type Mus musculus
Q170K pKa value 6.6 for N-oxygenation of 10-(N,N-dimethylaminooctyl)2-(trifluoromethyl)phenothiazene, compared with 6.9 for wild-type Homo sapiens
Q206H pKa value 6.5 for N-oxygenation of 10-(N,N-dimethylaminooctyl)2-(trifluoromethyl)phenothiazene, compared with 6.9 for wild-type Homo sapiens
Y228H pKa value 7.9 for N-oxygenation of 10-(N,N-dimethylaminooctyl)2-(trifluoromethyl)phenothiazene, compared with 6.9 for wild-type Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.117
-
 10-(N,N-dimethylaminooctyl)2-(trifluoromethyl)phenothiazine pH 8.4, 37°C Mus musculus

Organism

Organism UniProt Comment Textmining
Homo sapiens P49326
-
-
Mus musculus P97872 isoform FMO5
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Homo sapiens
recombinant protein Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-(N,N-dimethylaminooctyl)2-(trifluoromethyl)phenothiazene + NADPH + H+ + O2
-
 Homo sapiens ? + NADP+ + H2O
-
 ?
10-(N,N-dimethylaminooctyl)2-(trifluoromethyl)phenothiazene + NADPH + H+ + O2
-
 Mus musculus ? + NADP+ + H2O
-
 ?
10-(N,N-dimethylaminooctyl)2-(trifluoromethyl)phenothiazine + NADPH + H+ + O2
-
 Mus musculus 10-(N,N-dimethylaminooctyl)2-(trifluoromethyl)phenothiazine N-oxide + NADP+ + H2O
-
 r

Synonyms

Synonyms Comment Organism
flavin-containing monooxygenase 5
-
 Homo sapiens
flavin-containing monooxygenase 5
-
 Mus musculus

pH Range

pH Minimum pH Maximum Comment Organism
6 11
-
 Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
 almost complete loss of activity Mus musculus