BRENDA - Enzyme Database show
show all sequences of 1.14.13.79

OsWOX3A is involved in negative feedback regulation of the gibberellic acid biosynthetic pathway in rice (Oryza sativa)

Cho, S.H.; Kang, K.; Lee, S.H.; Lee, I.J.; Paek, N.C.; J. Exp. Bot. 67, 1677-1687 (2016)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene KAO encoding ent-kaurenoic acid oxidase, quantitative RT-PCR enzyme expression analysis
Oryza sativa Japonica Group
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ent-kaur-16-en-19-oate + 3 NADPH + 3 H+ + 3 O2
Oryza sativa Japonica Group
overall reaction
gibberellin A12 + 3 NADP+ + 4 H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Oryza sativa Japonica Group
Q33DK1
cv. Dongjinbye
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
KAO is broadly expressed in many organs and tissues
Oryza sativa Japonica Group
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ent-kaur-16-en-19-oate + 3 NADPH + 3 H+ + 3 O2
overall reaction
745468
Oryza sativa Japonica Group
gibberellin A12 + 3 NADP+ + 4 H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Oryza sativa Japonica Group
NADPH
-
Oryza sativa Japonica Group
Cloned(Commentary) (protein specific)
Commentary
Organism
gene KAO encoding ent-kaurenoic acid oxidase, quantitative RT-PCR enzyme expression analysis
Oryza sativa Japonica Group
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Oryza sativa Japonica Group
NADPH
-
Oryza sativa Japonica Group
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ent-kaur-16-en-19-oate + 3 NADPH + 3 H+ + 3 O2
Oryza sativa Japonica Group
overall reaction
gibberellin A12 + 3 NADP+ + 4 H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
KAO is broadly expressed in many organs and tissues
Oryza sativa Japonica Group
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ent-kaur-16-en-19-oate + 3 NADPH + 3 H+ + 3 O2
overall reaction
745468
Oryza sativa Japonica Group
gibberellin A12 + 3 NADP+ + 4 H2O
-
-
-
?
Expression
Organism
Commentary
Expression
Oryza sativa Japonica Group
OsWOX3A interacts directly with the KAO promoter to repress KAO expression. OsWOX3A interacts with a WOX-binding motif, TTAATCG, in the KAO promoter, mechanism modeling, overview. OsWOX3A is involved in the negative feedback regulation of KAO expression for gibberellic acid homeostasis
down
General Information
General Information
Commentary
Organism
malfunction
the rice mutant of KAO (oskao-1) has low levels of GA53, GA20, and GA1, OsWOX3A-OX downregulates KAO expression, which causes significantly reduced levels of GA53, GA20, and, finally, bioactive GA1. Quantitative analysis of gibberellic acid intermediates reveals significantly reduced levels of GA20 and bioactive GA1 in transgenic rice overexpressing OsWOX3A-OX, due to downregulation of the expression of KAO. The mutant OsWOX3A-OX exhibits severe dwarfism with very short and wide leaves, a phenotype that resembles that of gibberellic acid (GA)-deficient or GA-insensitive mutants. Loss of OsWOX3A function in narrow leaf2 (nal2) nal3 double mutants (termed nal2/3) causes pleiotropic effects, such as narrow and curly leaves, opened spikelets, narrow grains, more tillers, and fewer lateral roots, but almost normal plant height. Loss-of-function nal2/3 mutants show increased expression of KAO, changes in gibberellic acid contents are consistent with the expression levels of KAO and GA20ox2 in nal2/3 mutants
Oryza sativa Japonica Group
additional information
OsWOX3A acts as a transcriptional repressor, generation of transgenic rice overexpressing OsWOX3A, OsWOX3A-OX, the mutant consistently exhibits severe dwarfism with very short and wide leaves, a phenotype that resembles that of gibberellic acid (GA)-deficient or GA-insensitive mutants. OsWOX3A expression is drastically and temporarily upregulated by gibberellin GA3 and is downregulated by paclobutrazol, a blocker of gibberellic acid biosynthesis
Oryza sativa Japonica Group
physiological function
ent-kaurenoic acid oxidase, encoded by gene KAO, is a gibberellin biosynthetic enzyme. WUSCHEL-related homeobox 3 in Oryza sativa, OsWOX3A, is a gibberellic acid-responsive gene and functions in the negative feedback regulation of the gibberellic acid biosynthetic pathway for gibberellic acid homeostasis to maintain the threshold levels of endogenous gibberellic acid intermediates throughout development. OsWOX3A interacts directly with the KAO promoter to repress KAO expression. Changes in gibberellic acid contents are consistent with the expression levels of KAO and GA20ox2 in nal2/3 mutants. OsWOX3A directly downregulates the expression of KAO as a trans-repressor or upregulates the expression of GA20ox2 as a trans-activator
Oryza sativa Japonica Group
General Information (protein specific)
General Information
Commentary
Organism
malfunction
the rice mutant of KAO (oskao-1) has low levels of GA53, GA20, and GA1, OsWOX3A-OX downregulates KAO expression, which causes significantly reduced levels of GA53, GA20, and, finally, bioactive GA1. Quantitative analysis of gibberellic acid intermediates reveals significantly reduced levels of GA20 and bioactive GA1 in transgenic rice overexpressing OsWOX3A-OX, due to downregulation of the expression of KAO. The mutant OsWOX3A-OX exhibits severe dwarfism with very short and wide leaves, a phenotype that resembles that of gibberellic acid (GA)-deficient or GA-insensitive mutants. Loss of OsWOX3A function in narrow leaf2 (nal2) nal3 double mutants (termed nal2/3) causes pleiotropic effects, such as narrow and curly leaves, opened spikelets, narrow grains, more tillers, and fewer lateral roots, but almost normal plant height. Loss-of-function nal2/3 mutants show increased expression of KAO, changes in gibberellic acid contents are consistent with the expression levels of KAO and GA20ox2 in nal2/3 mutants
Oryza sativa Japonica Group
additional information
OsWOX3A acts as a transcriptional repressor, generation of transgenic rice overexpressing OsWOX3A, OsWOX3A-OX, the mutant consistently exhibits severe dwarfism with very short and wide leaves, a phenotype that resembles that of gibberellic acid (GA)-deficient or GA-insensitive mutants. OsWOX3A expression is drastically and temporarily upregulated by gibberellin GA3 and is downregulated by paclobutrazol, a blocker of gibberellic acid biosynthesis
Oryza sativa Japonica Group
physiological function
ent-kaurenoic acid oxidase, encoded by gene KAO, is a gibberellin biosynthetic enzyme. WUSCHEL-related homeobox 3 in Oryza sativa, OsWOX3A, is a gibberellic acid-responsive gene and functions in the negative feedback regulation of the gibberellic acid biosynthetic pathway for gibberellic acid homeostasis to maintain the threshold levels of endogenous gibberellic acid intermediates throughout development. OsWOX3A interacts directly with the KAO promoter to repress KAO expression. Changes in gibberellic acid contents are consistent with the expression levels of KAO and GA20ox2 in nal2/3 mutants. OsWOX3A directly downregulates the expression of KAO as a trans-repressor or upregulates the expression of GA20ox2 as a trans-activator
Oryza sativa Japonica Group
Expression (protein specific)
Organism
Commentary
Expression
Oryza sativa Japonica Group
OsWOX3A interacts directly with the KAO promoter to repress KAO expression. OsWOX3A interacts with a WOX-binding motif, TTAATCG, in the KAO promoter, mechanism modeling, overview. OsWOX3A is involved in the negative feedback regulation of KAO expression for gibberellic acid homeostasis
down
Other publictions for EC 1.14.13.79
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745468
Cho
OsWOX3A is involved in negati ...
Oryza sativa Japonica Group
J. Exp. Bot.
67
1677-1687
2016
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746078
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The gibberellin biosynthetic ...
Arabidopsis thaliana
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80
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9
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4
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15
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8
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