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Literature summary for 1.14.13.7 extracted from

  • Das, B.; Patra, S.
    Multisubstrate specific flavin containing monooxygenase from Chlorella pyrenoidosa with potential application for phenolic wastewater remediation and biosensor application (2018), Environ. Technol., 39, 2073-2089 .
    View publication on PubMed
Search for more literature for 1.14.13.7

Application

Application Comment Organism
environmental protection application for enzyme-based remediation of phenolic wastewater or in phenolic biosensor, kinetic properties. Remediation of phenols at its anthropogenic source before it enters into the environmental ecosystem. Microbial degradation has gained attention for treatment of phenols owing to its ability of complete mineralization and cost effectiveness Auxenochlorella pyrenoidosa

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 74.36% inhibition at 0.03 mM Auxenochlorella pyrenoidosa
Cu2+ 82.05% inhibition at 0.03 mM Auxenochlorella pyrenoidosa
Fe2+ 84.61% inhibition at 0.03 mM Auxenochlorella pyrenoidosa
H2O2 about 80% inhibition at 20% H2O2 Auxenochlorella pyrenoidosa
Hg2+ 82.05% inhibition at 0.03 mM Auxenochlorella pyrenoidosa
additional information Fe2+ chelators (phenanthroline and sodium arsenate), heavy metals, denaturants and oxidizing agents show inhibitory effect on phenol hydroxylation activity of the enzyme. Sodium diethyldithiocarbamte (Cu chelator) has no inhibitory effect on phenol hydroxylation activity Auxenochlorella pyrenoidosa
Phenanthroline 42.31% inhibition at 0.03 mM Auxenochlorella pyrenoidosa
SDS about 80% inhibition at 1 M SDS Auxenochlorella pyrenoidosa
Sodium arsenate 84.62% inhibition at 0.03 mM Auxenochlorella pyrenoidosa
Urea about 75% inhibition at 3 M urea Auxenochlorella pyrenoidosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic properties of partially purified enzyme, Michealis-Menten kinetics, overview Auxenochlorella pyrenoidosa
0.00171
-
 phenol pH 7.0, 35°C Auxenochlorella pyrenoidosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phenol + NADPH + H+ + O2 Auxenochlorella pyrenoidosa
-
 catechol + NADP+ + H2O
-
 ?
phenol + NADPH + H+ + O2 Auxenochlorella pyrenoidosa NCIM 2738
-
 catechol + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Auxenochlorella pyrenoidosa
-
-
-
Auxenochlorella pyrenoidosa NCIM 2738
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme partially by ammonium sulfate fractionation and dialysis, cost-effective production of partially purified microalgal phenol hydroylase by single-step purification Auxenochlorella pyrenoidosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.39
-
 partially purified enzyme, substrate phenol, pH 7.0, 35°C Auxenochlorella pyrenoidosa

Storage Stability

Storage Stability Organism
the enzyme shows remarkable storage stability at room temperature and at 4°C, minimal loss of 5.13% and 1.28% after storage of 7 days at room temperature and 4°C, respectively Auxenochlorella pyrenoidosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dichlorophenol + NADPH + H+ + O2
-
 Auxenochlorella pyrenoidosa 2,4-dichlorocatechol + NADP+ + H2O
-
 ?
2,4-dichlorophenol + NADPH + H+ + O2
-
 Auxenochlorella pyrenoidosa NCIM 2738 2,4-dichlorocatechol + NADP+ + H2O
-
 ?
2-aminophenol + NADPH + H+ + O2
-
 Auxenochlorella pyrenoidosa 2-aminocatechol + NADP+ + H2O
-
 ?
2-aminophenol + NADPH + H+ + O2
-
 Auxenochlorella pyrenoidosa NCIM 2738 2-aminocatechol + NADP+ + H2O
-
 ?
2-chlorophenol + NADPH + H+ + O2
-
 Auxenochlorella pyrenoidosa 2-chlorocatechol + NADP+ + H2O
-
 ?
2-chlorophenol + NADPH + H+ + O2
-
 Auxenochlorella pyrenoidosa NCIM 2738 2-chlorocatechol + NADP+ + H2O
-
 ?
2-cresol + NADPH + H+ + O2
-
 Auxenochlorella pyrenoidosa ? + NADP+ + H2O
-
 ?
3-aminophenol + NADPH + H+ + O2
-
 Auxenochlorella pyrenoidosa 3-aminocatechol + NADP+ + H2O
-
 ?
3-cresol + NADPH + H+ + O2
-
 Auxenochlorella pyrenoidosa ? + NADP+ + H2O
-
 ?
3-hydroxybenzaldehyde + NADPH + H+ + O2
-
 Auxenochlorella pyrenoidosa ? + NADP+ + H2O
-
 ?
4-bromophenol + NADPH + H+ + O2
-
 Auxenochlorella pyrenoidosa 4-bromocatechol + NADP+ + H2O
-
 ?
4-chlorophenol + NADPH + H+ + O2
-
 Auxenochlorella pyrenoidosa 4-chlorocatechol + NADP+ + H2O
-
 ?
4-cresol + NADPH + H+ + O2
-
 Auxenochlorella pyrenoidosa ? + NADP+ + H2O
-
 ?
4-hydroxybenzaldehyde + NADPH + H+ + O2
-
 Auxenochlorella pyrenoidosa ? + NADP+ + H2O
-
 ?
4-hydroxybenzoate + NADPH + H+ + O2
-
 Auxenochlorella pyrenoidosa ? + NADP+ + H2O
-
 ?
4-nitrophenol + NADPH + H+ + O2
-
 Auxenochlorella pyrenoidosa 4-nitrocatechol + NADP+ + H2O
-
 ?
catechol + NADPH + H+ + O2
-
 Auxenochlorella pyrenoidosa ? + NADP+ + H2O
-
 ?
additional information the enzyme has broad substrate specificity against isomeric diphenols, isomeric methylphenols, halogen-substituted phenols, amino-substituted phenols, nitrophenols, hydroxybenzaldehyde and hydroxylbenzoic acid Auxenochlorella pyrenoidosa ?
-
-
additional information the enzyme has broad substrate specificity against isomeric diphenols, isomeric methylphenols, halogen-substituted phenols, amino-substituted phenols, nitrophenols, hydroxybenzaldehyde and hydroxylbenzoic acid Auxenochlorella pyrenoidosa NCIM 2738 ?
-
-
phenol + NADPH + H+ + O2
-
 Auxenochlorella pyrenoidosa catechol + NADP+ + H2O
-
 ?
phenol + NADPH + H+ + O2
-
 Auxenochlorella pyrenoidosa NCIM 2738 catechol + NADP+ + H2O
-
 ?
quinol + NADPH + H+ + O2
-
 Auxenochlorella pyrenoidosa ? + NADP+ + H2O
-
 ?
resorcinol + NADPH + H+ + O2
-
 Auxenochlorella pyrenoidosa ? + NADP+ + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 25000 Auxenochlorella pyrenoidosa
More peptide mass fingerprinting and MALDI-TOF analysis of the 25 kD enzyme Auxenochlorella pyrenoidosa

Synonyms

Synonyms Comment Organism
flavin containing monooxygenase
-
 Auxenochlorella pyrenoidosa
phenol hydroxylase
-
 Auxenochlorella pyrenoidosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Auxenochlorella pyrenoidosa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 85 activity range, profile overview Auxenochlorella pyrenoidosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Auxenochlorella pyrenoidosa

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8 activity range, profile overview. Over 50% of maximal activity at pH 5.5-7.5 Auxenochlorella pyrenoidosa

Cofactor

Cofactor Comment Organism Structure
additional information phenol hydroxylase shows specific ability to use NADH or NADPH or both as cofactors, phenol is hydroxylated to catechol with concomitant oxidation of NADH. The specific activity of phenol hydroxylase reaction with NADPH as cofactor is about 3times higher when compared to specific activity with NADH as cofactor, suggesting NADPH is a better cofactor Auxenochlorella pyrenoidosa
NADPH
-
 Auxenochlorella pyrenoidosa