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Literature summary for 1.14.13.7 extracted from

  • Xiao, X.; Si, M.; Yang, Z.; Zhang, Y.; Guan, J.; Chaudhry, M.T.; Wang, Y.; Shen, X.
    Molecular characterization of a eukaryotic-like phenol hydroxylase from Corynebacterium glutamicum (2015), J. Gen. Appl. Microbiol., 61, 99-107 .
    View publication on PubMed
Search for more literature for 1.14.13.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Corynebacterium glutamicum

Protein Variants

Protein Variants Comment Organism
C349S the mutant shows 30% activity compared to the wild type enzyme Corynebacterium glutamicum
C476S the mutant shows 45% activity compared to the wild type enzyme Corynebacterium glutamicum
D75N the mutant shows 288% activity compared to the wild type enzyme Corynebacterium glutamicum
P261S the mutant shows 15% activity compared to the wild type enzyme Corynebacterium glutamicum
R262S the mutant shows 427% activity compared to the wild type enzyme Corynebacterium glutamicum
R269L the mutant shows 232% activity compared to the wild type enzyme Corynebacterium glutamicum

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 63% residual activity at 1 mM Corynebacterium glutamicum
Cu2+ 37% residual activity at 0.02 mM Corynebacterium glutamicum
Fe2+ complete inhibition at 1 mM Corynebacterium glutamicum
Fe3+ 3% residual activity at 1 mM Corynebacterium glutamicum
Ni2+ 4% residual activity at 1 mM Corynebacterium glutamicum
Zn2+ 13% residual activity at 1 mM Corynebacterium glutamicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.256
-
 phenol at pH 8.0 and 45°C Corynebacterium glutamicum
0.284
-
 NADPH at pH 8.0 and 45°C Corynebacterium glutamicum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 119% activity at 1 mM Corynebacterium glutamicum
Mn2+ 121% activity at 1 mM Corynebacterium glutamicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
140000
-
 gel filtration Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phenol + NADPH + H+ + O2 Corynebacterium glutamicum
-
 catechol + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-bind Ni-NTA resin column chromatography Corynebacterium glutamicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,3-dihydroxybenzene + NADPH + H+ + O2 50.12% activity compared to phenol Corynebacterium glutamicum ? + NADP+ + H2O
-
 ?
2-hydroxybenzoic acid + NADPH + H+ + O2 15.97% activity compared to phenol Corynebacterium glutamicum ? + NADP+ + H2O
-
 ?
2-naphthol + NADPH + H+ + O2 9.75% activity compared to phenol Corynebacterium glutamicum ? + NADP+ + H2O
-
 ?
3-hydroxybenzoic acid + NADPH + H+ + O2 18.53% activity compared to phenol Corynebacterium glutamicum ? + NADP+ + H2O
-
 ?
4-chlorophenol + NADPH + H+ + O2 28.6% activity compared to phenol Corynebacterium glutamicum ? + NADP+ + H2O
-
 ?
4-hydroxybenzoic acid + NADPH + H+ + O2 14.88% activity compared to phenol Corynebacterium glutamicum ? + NADP+ + H2O
-
 ?
hydroquinone + NADPH + H+ + O2 48.25% activity compared to phenol Corynebacterium glutamicum ? + NADP+ + H2O
-
 ?
phenol + NADH + H+ + O2 activity is reduced by 84% when NADPH is replaced by NADH Corynebacterium glutamicum catechol + NAD+ + H2O
-
 ?
phenol + NADPH + H+ + O2
-
 Corynebacterium glutamicum catechol + NADP+ + H2O
-
 ?
phenol + NADPH + H+ + O2 100% activity Corynebacterium glutamicum catechol + NADP+ + H2O
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 70000, SDS-PAGE Corynebacterium glutamicum

Synonyms

Synonyms Comment Organism
NCgl2588
-
 Corynebacterium glutamicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Corynebacterium glutamicum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 50 more than 50% activity between 40 and 50°C Corynebacterium glutamicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Corynebacterium glutamicum

pH Range

pH Minimum pH Maximum Comment Organism
7 9 more than 80% activity between pH 7.0 and 9.0 Corynebacterium glutamicum

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Corynebacterium glutamicum
NADPH the enzyme has a preference for NADPH as a cofactor Corynebacterium glutamicum

pI Value

Organism Comment pI Value Maximum pI Value
Corynebacterium glutamicum calculated from amino acid sequence
-
 4.9