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Literature summary for 1.14.13.64 extracted from

  • Shen, X.; Wang, J.; Wang, J.; Chen, Z.; Yuan, Q.; Yan, Y.
    High-level de novo biosynthesis of arbutin in engineered Escherichia coli (2017), Metab. Eng., 42, 52-58 .
    View publication on PubMed

Application

Application Comment Organism
pharmacology construction of a novel artificial pathway for arbutin biosynthesis in Escherichia colid. De novo biosynthesis of arbutin from simple carbon sources is established and a generalizable strategy for the biosynthesis of shikimate pathway derived chemicals is provided. Arbutin is a hydroquinone glucoside compound existing in various plants. It is widely used in pharmaceuticaland cosmetic industries owing to its well-known skin-lightening property as well as anti-oxidant, anti-microbial, and anti-inflammatory activities. A 4-hydroxybenzoate 1-hydroxylase gene from Candida parapsilosis CBS604 and a glucosyltransferase (arbutin synthase) gene from Rauvolfia serpentina are introduced into Escherichia coli lead to the production of 54.71 mg/l of arbutin from glucose. Further redirection of carbon flux into arbutin biosynthesis pathway by enhancing shikimate pathway genes enables production of 3.29 g/l arbutin, which is a 60-fold increase compared with the initial strain. Final optimization of glucose concentration added in the culture medium is able to further improve the titer of arbutin to 4.19 g/l in shake flasks experiments, which is around 77-fold higher than that of initial strain Candida parapsilosis
synthesis construction of a novel artificial pathway for arbutin biosynthesis in Escherichia colid. De novo biosynthesis of arbutin from simple carbon sources is established and a generalizable strategy for the biosynthesis of shikimate pathway derived chemicals is provided. Arbutin is a hydroquinone glucoside compound existing in various plants. It is widely used in pharmaceutical and cosmetic industries owing to its well-known skin-lightening property as well as anti-oxidant, anti-microbial, and anti-inflammatory activities. A 4-hydroxybenzoate 1-hydroxylase gene from Candida parapsilosis CBS604 and a glucosyltransferase (arbutin synthase) gene from Rauvolfia serpentina are introduced into Escherichia coli lead to the production of 54.71 mg/l of arbutin from glucose. Further redirection of carbon flux into arbutin biosynthesis pathway by enhancing shikimate pathway genes enables production of 3.29 g/l arbutin, which is a 60-fold increase compared with the initial strain. Final optimization of glucose concentration added in the culture medium is able to further improve the titer of arbutin to 4.19 g/l in shake flasks experiments, which is around 77-fold higher than that of initial strain Candida parapsilosis
synthesis an artificial pathway is established in Escherichia coli for high-level production of arbutin from simple carbon sources in Escherichia coli for high-level production of arbutin from simple carbon sources. Introduction of the genes for 4-hydroxybenzoate 1-hydroxylase from Candida parapsilosis CBS604 and hydroquinone glucosyltransferase from Rauvolfia serpentina into Escherichia coli leads to the production of 54.71 mg/l of arbutin from glucose. Further redirection of carbon flux into arbutin biosynthesis pathway by enhancing shikimate pathway genes enables production of 3.29 g/l arbutin. Final optimization of glucose concentration added in the culture medium is able to further improve the titer of arbutin to 4.19 g/l in shake flasks experiments Candida parapsilosis

Cloned(Commentary)

Cloned (Comment) Organism
a 4-hydroxybenzoate 1-hydroxylase gene from Candida parapsilosis CBS604 and a glucosyltransferase (arbutin synthase) gene from Rauvolfia serpentina are introduced into Escherichia coli lead to the production of 54.71 mg/l of arbutin from glucose Candida parapsilosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.63
-
4-hydroxybenzoate pH 7.5, 30°C Candida parapsilosis
0.63
-
4-hydroxybenzoate pH 7.6, 30°C Candida parapsilosis

Organism

Organism UniProt Comment Textmining
Candida parapsilosis
-
-
-
Candida parapsilosis G8B709
-
-
Candida parapsilosis CBS604
-
-
-
Candida parapsilosis CBS604 G8B709
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxybenzoate + NADH + 2 H+ + O2
-
Candida parapsilosis hydroquinone + NAD+ + H2O + CO2
-
?
4-hydroxybenzoate + NADH + 2 H+ + O2
-
Candida parapsilosis CBS604 hydroquinone + NAD+ + H2O + CO2
-
?

Synonyms

Synonyms Comment Organism
4-hydroxybenzoate 1-hydroxylase
-
Candida parapsilosis
MNX1
-
Candida parapsilosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Candida parapsilosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.136
-
4-hydroxybenzoate pH 7.5, 30°C Candida parapsilosis
0.136
-
4-hydroxybenzoate pH 7.6, 30°C Candida parapsilosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Candida parapsilosis
7.6
-
assay at Candida parapsilosis

Cofactor

Cofactor Comment Organism Structure
FAD required Candida parapsilosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.22
-
4-hydroxybenzoate pH 7.5, 30°C Candida parapsilosis
0.22
-
4-hydroxybenzoate pH 7.6, 30°C Candida parapsilosis