Application | Comment | Organism |
---|---|---|
pharmacology | construction of a novel artificial pathway for arbutin biosynthesis in Escherichia colid. De novo biosynthesis of arbutin from simple carbon sources is established and a generalizable strategy for the biosynthesis of shikimate pathway derived chemicals is provided. Arbutin is a hydroquinone glucoside compound existing in various plants. It is widely used in pharmaceuticaland cosmetic industries owing to its well-known skin-lightening property as well as anti-oxidant, anti-microbial, and anti-inflammatory activities. A 4-hydroxybenzoate 1-hydroxylase gene from Candida parapsilosis CBS604 and a glucosyltransferase (arbutin synthase) gene from Rauvolfia serpentina are introduced into Escherichia coli lead to the production of 54.71 mg/l of arbutin from glucose. Further redirection of carbon flux into arbutin biosynthesis pathway by enhancing shikimate pathway genes enables production of 3.29 g/l arbutin, which is a 60-fold increase compared with the initial strain. Final optimization of glucose concentration added in the culture medium is able to further improve the titer of arbutin to 4.19 g/l in shake flasks experiments, which is around 77-fold higher than that of initial strain | Candida parapsilosis |
synthesis | construction of a novel artificial pathway for arbutin biosynthesis in Escherichia colid. De novo biosynthesis of arbutin from simple carbon sources is established and a generalizable strategy for the biosynthesis of shikimate pathway derived chemicals is provided. Arbutin is a hydroquinone glucoside compound existing in various plants. It is widely used in pharmaceutical and cosmetic industries owing to its well-known skin-lightening property as well as anti-oxidant, anti-microbial, and anti-inflammatory activities. A 4-hydroxybenzoate 1-hydroxylase gene from Candida parapsilosis CBS604 and a glucosyltransferase (arbutin synthase) gene from Rauvolfia serpentina are introduced into Escherichia coli lead to the production of 54.71 mg/l of arbutin from glucose. Further redirection of carbon flux into arbutin biosynthesis pathway by enhancing shikimate pathway genes enables production of 3.29 g/l arbutin, which is a 60-fold increase compared with the initial strain. Final optimization of glucose concentration added in the culture medium is able to further improve the titer of arbutin to 4.19 g/l in shake flasks experiments, which is around 77-fold higher than that of initial strain | Candida parapsilosis |
synthesis | an artificial pathway is established in Escherichia coli for high-level production of arbutin from simple carbon sources in Escherichia coli for high-level production of arbutin from simple carbon sources. Introduction of the genes for 4-hydroxybenzoate 1-hydroxylase from Candida parapsilosis CBS604 and hydroquinone glucosyltransferase from Rauvolfia serpentina into Escherichia coli leads to the production of 54.71 mg/l of arbutin from glucose. Further redirection of carbon flux into arbutin biosynthesis pathway by enhancing shikimate pathway genes enables production of 3.29 g/l arbutin. Final optimization of glucose concentration added in the culture medium is able to further improve the titer of arbutin to 4.19 g/l in shake flasks experiments | Candida parapsilosis |
Cloned (Comment) | Organism |
---|---|
a 4-hydroxybenzoate 1-hydroxylase gene from Candida parapsilosis CBS604 and a glucosyltransferase (arbutin synthase) gene from Rauvolfia serpentina are introduced into Escherichia coli lead to the production of 54.71 mg/l of arbutin from glucose | Candida parapsilosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.63 | - |
4-hydroxybenzoate | pH 7.5, 30°C | Candida parapsilosis | |
0.63 | - |
4-hydroxybenzoate | pH 7.6, 30°C | Candida parapsilosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida parapsilosis | - |
- |
- |
Candida parapsilosis | G8B709 | - |
- |
Candida parapsilosis CBS604 | - |
- |
- |
Candida parapsilosis CBS604 | G8B709 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxybenzoate + NADH + 2 H+ + O2 | - |
Candida parapsilosis | hydroquinone + NAD+ + H2O + CO2 | - |
? | |
4-hydroxybenzoate + NADH + 2 H+ + O2 | - |
Candida parapsilosis CBS604 | hydroquinone + NAD+ + H2O + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
4-hydroxybenzoate 1-hydroxylase | - |
Candida parapsilosis |
MNX1 | - |
Candida parapsilosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Candida parapsilosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.136 | - |
4-hydroxybenzoate | pH 7.5, 30°C | Candida parapsilosis | |
0.136 | - |
4-hydroxybenzoate | pH 7.6, 30°C | Candida parapsilosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Candida parapsilosis |
7.6 | - |
assay at | Candida parapsilosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | required | Candida parapsilosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
4-hydroxybenzoate | pH 7.5, 30°C | Candida parapsilosis | |
0.22 | - |
4-hydroxybenzoate | pH 7.6, 30°C | Candida parapsilosis |