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Literature summary for 1.14.13.62 extracted from

  • Block, D.W.; Lingens, F.
    XIII. Purification and properties of 1H-4-oxoquinoline monooxygenase from Pseudomonas putida strain 33/1 (1992), Biol. Chem. Hoppe-Seyler, 373, 249-254.
    View publication on PubMed
Search for more literature for 1.14.13.62

Activating Compound

Activating Compound Comment Organism Structure
acetone 15%, 1.2fold activation. Strong decrease in activity above 15% Pseudomonas putida
ethanol 15%, 1.7fold activation. Strong decrease in activity above 15% Pseudomonas putida

General Stability

General Stability Organism
50% loss of enzyme activity after 96 h without additions Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate partial inhibition Pseudomonas putida
Cu2+
-
 Pseudomonas putida
H2O2
-
 Pseudomonas putida
Hg2+
-
 Pseudomonas putida
iodoacetate partial inhibition Pseudomonas putida
Sodium dithionite
-
 Pseudomonas putida
Zn2+
-
 Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
 1H-4-Oxoquinoline
-
 Pseudomonas putida
0.087
-
 NADH
-
 Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 3 * 42000, SDS-PAGE Pseudomonas putida
126000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas putida inducible by 1H-4-oxoquinoline ?
-
 ?
additional information Pseudomonas putida 33/1 inducible by 1H-4-oxoquinoline ?
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone 40% loss of activity after 2 h in 15% acetone Pseudomonas putida
Ethanol stable for 2 h in 15% ethanol, 50% loss of activity after 24 h Pseudomonas putida
Ethylene glycol completely stable after 96 h in 50% ethylene glycol Pseudomonas putida
additional information 50% loss of enzyme activity after 96 h without addition of organic solvents Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida 33/1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.8
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1H-4-Oxoquinoline + NADH + O2
-
 Pseudomonas putida 1H-3-Hydroxy-4-oxoquinoline + NAD+ + H2O
-
 ?
1H-4-Oxoquinoline + NADH + O2
-
 Pseudomonas putida 33/1 1H-3-Hydroxy-4-oxoquinoline + NAD+ + H2O
-
 ?
1H-4-Oxoquinoline + NADPH + O2 50% of the activity with NADH Pseudomonas putida 1H-3-Hydroxy-4-oxoquinoline + NADP+ + H2O
-
 ?
1H-4-Oxoquinoline + NADPH + O2 50% of the activity with NADH Pseudomonas putida 33/1 1H-3-Hydroxy-4-oxoquinoline + NADP+ + H2O
-
 ?
additional information inducible by 1H-4-oxoquinoline Pseudomonas putida ?
-
 ?
additional information inducible by 1H-4-oxoquinoline Pseudomonas putida 33/1 ?
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 42000, SDS-PAGE Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
 Pseudomonas putida

pH Stability

pH Stability pH Stability Maximum Comment Organism
8.5
-
 strong inactivation above Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NADH required as cofactor Pseudomonas putida
NADPH 50% of the activity compared to NADH Pseudomonas putida